Browsing Faculty of Arts and Sciences by Keyword "Developmental Biology"
Now showing items 1-20 of 31
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Ablation of a Single Cell From Eight-cell Embryos of the Amphipod Crustacean Parhyale hawaiensis
(MyJove Corporation, 2014)The amphipod Parhyale hawaiensis is a small crustacean found in intertidal marine habitats worldwide. Over the past decade, Parhyale has emerged as a promising model organism for laboratory studies of development, providing ... -
Analysis of the Effects of Five Factors Relevant to In Vitro Chondrogenesis of Human Mesenchymal Stem Cells Using Factorial Design and High Throughput mRNA-Profiling
(Public Library of Science, 2014)The in vitro process of chondrogenic differentiation of mesenchymal stem cells for tissue engineering has been shown to require three-dimensional culture along with the addition of differentiation factors to the culture ... -
Bioelectric Signaling Regulates Size in Zebrafish Fins
(Public Library of Science, 2014)The scaling relationship between the size of an appendage or organ and that of the body as a whole is tightly regulated during animal development. If a structure grows at a different rate than the rest of the body, this ... -
Comparison and Optimization of hiPSC Forebrain Cortical Differentiation Protocols
(Public Library of Science, 2014)Several protocols have been developed for human induced pluripotent stem cell neuronal differentiation. We compare several methods for forebrain cortical neuronal differentiation by assessing cell morphology, immunostaining ... -
A Comprehensive Reference Transcriptome Resource for the Common House Spider Parasteatoda tepidariorum
(Public Library of Science, 2014)Parasteatoda tepidariorum is an increasingly popular model for the study of spider development and the evolution of development more broadly. However, fully understanding the regulation and evolution of P. tepidariorum ... -
Derivation of Corneal Keratocyte-Like Cells from Human Induced Pluripotent Stem Cells
(Public Library of Science, 2016)Corneal diseases such as keratoconus represent a relatively common disorder in the human population. However, treatment is restricted to corneal transplantation, which only occurs in the most advanced cases. Cell based ... -
Distinct Contributions of Astrocytes and Pericytes to Neuroinflammation Identified in a 3D Human Blood-Brain Barrier on a Chip
(Public Library of Science, 2016)Neurovascular inflammation is a major contributor to many neurological disorders, but modeling these processes in vitro has proven to be difficult. Here, we microengineered a three-dimensional (3D) model of the human ... -
The Evolution of Cell-to-Cell Communication in a Sporulating Bacterium
(Public Library of Science, 2012)Traditionally microorganisms were considered to be autonomous organisms that could be studied in isolation. However, over the last decades cell-to-cell communication has been found to be ubiquitous. By secreting molecular ... -
Fibroblasts Derived from Human Pluripotent Stem Cells Activate Angiogenic Responses In Vitro and In Vivo
(Public Library of Science, 2013)Human embryonic and induced pluripotent stem cells (hESC/hiPSC) are promising cell sources for the derivation of large numbers of specific cell types for tissue engineering and cell therapy applications. We have describe ... -
FRAP Analysis: Accounting for Bleaching during Image Capture
(Public Library of Science, 2012)The analysis of Fluorescence Recovery After Photobleaching (FRAP) experiments involves mathematical modeling of the fluorescence recovery process. An important feature of FRAP experiments that tends to be ignored in the ... -
From Clinging to Digging: The Postembryonic Skeletal Ontogeny of the Indian Purple Frog, Nasikabatrachus sahyadrensis (Anura: Nasikabatrachidae)
(Public Library of Science, 2016)The Indian Purple frog, Nasikabatrachus sahyadrensis, occupies a basal phylogenetic position among neobatrachian anurans and has a very unusual life history. Tadpoles have a large ventral oral sucker, which they use to ... -
Functional morphology of the ankle extensor muscle‐tendon units in the springhare Pedetes capensis shows convergent evolution with macropods for bipedal hopping locomotion
(Wiley, 2020-06-25)This study assesses the functional morphology of the ankle extensor muscle-tendon units of the springhare Pedetes capensis, an African bipedal hopping rodent, to test for convergent evolution with the Australian bipedal ... -
Germ Cells Are Not Required to Establish the Female Pathway in Mouse Fetal Gonads
(Public Library of Science, 2012)The fetal gonad is composed of a mixture of somatic cell lineages and germ cells. The fate of the gonad, male or female, is determined by a population of somatic cells that differentiate into Sertoli or granulosa cells and ... -
Heads, Shoulders, Elbows, Knees, and Toes: Modular Gdf5 Enhancers Control Different Joints in the Vertebrate Skeleton
(Public Library of Science, 2016)Synovial joints are crucial for support and locomotion in vertebrates, and are the frequent site of serious skeletal defects and degenerative diseases in humans. Growth and differentiation factor 5 (Gdf5) is one of the ... -
Identification of a Novel Link between the Protein Kinase NDR1 and TGFβ Signaling in Epithelial Cells
(Public Library of Science, 2013)Transforming growth factor-beta (TGFβ) is a secreted polypeptide that plays essential roles in cellular development and homeostasis. Although mechanisms of TGFβ-induced responses have been characterized, our understanding ... -
An Insulin-to-Insulin Regulatory Network Orchestrates Phenotypic Specificity in Development and Physiology
(Public Library of Science, 2014)Insulin-like peptides (ILPs) play highly conserved roles in development and physiology. Most animal genomes encode multiple ILPs. Here we identify mechanisms for how the forty Caenorhabditis elegans ILPs coordinate diverse ... -
Interrogating the differential roles of Ezh1/2 in hematopoietic stem and progenitor cell formation from endothelial precursors
(2021-09-09)Understanding how hematopoietic stem and progenitor cells (HSPCs) are specified from endothelial precursors is essential to the goal of generating patient-specific HSPCs for therapeutic use. Coordinated action of cell ... -
Intraductal Injection for Localized Drug Delivery to the Mouse Mammary Gland
(MyJove Corporation, 2013)Herein we describe a protocol to deliver various reagents to the mouse mammary gland via intraductal injections. Localized drug delivery and knock-down of genes within the mammary epithelium has been difficult to achieve ... -
The Isolation, Differentiation, and Survival In Vivo of Multipotent Cells from the Postnatal Rat filum terminale
(Public Library of Science, 2013)Neural stem cells (NSCs) are undifferentiated cells in the central nervous system (CNS) that are capable of self-renewal and can be induced to differentiate into neurons and glia. Current sources of mammalian NSCs are ... -
The Kinematics of Plant Nutation Reveals a Simple Relation between Curvature and the Orientation of Differential Growth
(Public Library of Science, 2016)Nutation is an oscillatory movement that plants display during their development. Despite its ubiquity among plants movements, the relation between the observed movement and the underlying biological mechanisms remains ...