Browsing Faculty of Arts and Sciences by Keyword "Genetic Engineering"

Now showing items 1-4 of 4

    • Angiotensin II Induced Cardiac Dysfunction on a Chip 

      Horton, Renita E.; Yadid, Moran; McCain, Megan L.; Sheehy, Sean P.; Pasqualini, Francesco S.; Park, Sung-Jin; Cho, Alexander; Campbell, Patrick; Parker, Kevin Kit (Public Library of Science, 2016)
      In vitro disease models offer the ability to study specific systemic features in isolation to better understand underlying mechanisms that lead to dysfunction. Here, we present a cardiac dysfunction model using angiotensin ...

    • Development of an Optimized Medium, Strain and High-Throughput Culturing Methods for Methylobacterium extorquens 

      Delaney, Nigel Francis; Kaczmarek, Maria E.; Ward, Lewis M.; Swanson, Paige Kathleen; Lee, Ming-Chun; Marx, Christopher J (Public Library of Science, 2013)
      Methylobacterium extorquens strains are the best-studied methylotrophic model system, and their metabolism of single carbon compounds has been studied for over 50 years. Here we develop a new system for high-throughput ...

    • Efficient Mutagenesis by Cas9 Protein-Mediated Oligonucleotide Insertion and Large-Scale Assessment of Single-Guide RNAs 

      Gagnon, James A.; Valen, Eivind; Thyme, Summer B.; Huang, Peng; Ahkmetova, Laila; Pauli, Andrea; Montague, Tessa G.; Zimmerman, Steven; Richter, Constance; Schier, Alexander F. (Public Library of Science, 2014)
      The CRISPR/Cas9 system has been implemented in a variety of model organisms to mediate site-directed mutagenesis. A wide range of mutation rates has been reported, but at a limited number of genomic target sites. To uncover ...

    • High throughput functional variant screens via in-vivo production of single-stranded DNA 

      Schubert, Max Gabriel (2020-11-23)
      Tremendous genetic variation exists in nature, but our ability to create and characterize individual genetic variants remains far more limited in scale. Likewise, engineering proteins and phenotypes requires the introduction ...