Class II MHC Self-Antigen Presentation in Human B and T Lymphocytes
View/ Open
Published Version
https://doi.org/10.1371/journal.pone.0029805Metadata
Show full item recordCitation
Costantino, Cristina Maria, Eric Spooner, Hidde L. Ploegh, and David A. Hafler. 2012. Class II MHC self-antigen presentation in human B and T lymphocytes. PLoS ONE 7(1): e29805.Abstract
Human CD4\(^+\) T cells process and present functional class II MHC-peptide complexes, but the endogenous peptide repertoire of these non-classical antigen presenting cells remains unknown. We eluted and sequenced HLA-DR-bound self-peptides presented by CD4\(^+\) T cells in order to compare the T cell-derived peptide repertoire to sequences derived from genetically identical B cells. We identified several novel epitopes derived from the T cell-specific proteome, including fragments of CD4 and IL-2. While these data confirm that T cells can present peptides derived from the T-cell specific proteome, the vast majority of peptides sequenced after elution from MHC were derived from the common proteome. From this pool, we identified several identical peptide epitopes in the T and B cell repertoire derived from common endogenous proteins as well as novel endogenous epitopes with promiscuous binding. These findings indicate that the endogenous HLA-DR-bound peptide repertoire, regardless of APC type and across MHC isotype, is largely derived from the same pool of self-protein.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3267721/pdf/Terms of Use
This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAACitable link to this page
http://nrs.harvard.edu/urn-3:HUL.InstRepos:10288453
Collections
- HMS Scholarly Articles [17917]
Contact administrator regarding this item (to report mistakes or request changes)