Functional Multiplex Reporter Assay Using Tagged Gaussia Luciferase
View/ Open
Author
van Rijn, Sjoerd
Nilsson, Jonas
Noske, David P.
Vandertop, W. Peter
Würdinger, Thomas
Note: Order does not necessarily reflect citation order of authors.
Published Version
https://doi.org/10.1038/srep01046Metadata
Show full item recordCitation
Van Rijn, Sjoerd, Jonas Nilsson, David P. Noske, W. Peter Vandertop, Bakhos A. Tannous, and Thomas Würdinger. 2013. Functional multiplex reporter assay using tagged Gaussia luciferase. Scientific Reports 3: 1046.Abstract
We have developed a multiplex reporter system to monitor multiple biological variables in real-time. The secreted Gaussia luciferase was fused to ten different epitope tags (Gluc\(_{tag}\)), each expressed in different tumor cells. By immunobinding of the tags followed by Gluc\(_{tag}\) detection, this system allowed the independent and real-time monitoring of mixed cell cultures in vitro and of mixed subcutaneous and intracranial tumor subpopulations in vivo.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3541509/pdf/Terms of Use
This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAACitable link to this page
http://nrs.harvard.edu/urn-3:HUL.InstRepos:10622999
Collections
- HMS Scholarly Articles [17922]
Contact administrator regarding this item (to report mistakes or request changes)