Detection of JC Virus-Specific Immune Responses in a Novel Humanized Mouse Model
View/ Open
Author
Broge, Thomas A.
Vrbanac, Vladimir
Viscidi, Raphael
Gordon, Jennifer
Published Version
https://doi.org/10.1371/journal.pone.0064313Metadata
Show full item recordCitation
Tan, Chen Sabrina, Thomas A. Broge, Edward Seung, Vlad Vrbanac, Raphael Viscidi, Jennifer Gordon, Andrew M. Tager, and Igor J. Koralnik. 2013. Detection of jc virus-specific immune responses in a novel humanized mouse model. PLoS ONE 8(5): e64313.Abstract
Progressive Multifocal Leukoencephalopathy (PML) is an often fatal disease caused by the reactivation of the JC virus (JCV). Better understanding of viral-host interactions has been hampered by the lack of an animal model. Engrafting NOD/SCID/IL-2-Rg (null) mice with human lymphocytes and thymus, we generated a novel animal model for JCV infection. Mice were inoculated with either a PML isolate, JCV Mad-4, or with JCV CY, found in the kidney and urine of healthy individuals. While mice remained asymptomatic following inoculation, JCV DNA was occasionally detected in both the blood and the urine compartments. Mice generated both humoral and cellular immune responses against JCV. Expressions of immune exhaustion marker, PD-1, on lymphocytes were consistent with response to infection. Using this model we present the first in vivo demonstration of virological and immunological differences between JCV Mad-4 and CY. This model may prove valuable for studying JCV host immune responses.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3658964/pdf/Terms of Use
This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAACitable link to this page
http://nrs.harvard.edu/urn-3:HUL.InstRepos:11177930
Collections
- HMS Scholarly Articles [17918]
Contact administrator regarding this item (to report mistakes or request changes)