Reprogramming within Hours Following Nuclear Transfer into Mouse but not Human Zygotes
Author
Egli, Dieter
Chen, Alice E.
Ichida, Justin
Fitzgerald, Claire
Go, Kathryn J.
Acevedo, Nicole
Patel, Jay
Kearns, William G.
Goland, Robin
Leibel, Rudolph L.
Note: Order does not necessarily reflect citation order of authors.
Published Version
https://doi.org/10.1038/ncomms1503Metadata
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Egli, Dieter, Alice E. Chen, Genevieve Saphier, Justin Ichida, Claire Fitzgerald, Kathryn J. Go, Nicole Acevedo, et al. 2011. Reprogramming within hours following nuclear transfer into mouse but not human zygotes. Nature Communications 2: 488.Abstract
Fertilized mouse zygotes can reprogram somatic cells to a pluripotent state. Human zygotes might therefore be useful for producing patient-derived pluripotent stem cells. However, logistical, legal and social considerations have limited the availability of human eggs for research. Here we show that a significant number of normal fertilized eggs (zygotes) can be obtained for reprogramming studies. Using these zygotes, we found that when the zygotic genome was replaced with that of a somatic cell, development progressed normally throughout the cleavage stages, but then arrested before the morula stage. This arrest was associated with a failure to activate transcription in the transferred somatic genome. In contrast to human zygotes, mouse zygotes reprogrammed the somatic cell genome to a pluripotent state within hours after transfer. Our results suggest that there may be a previously unappreciated barrier to successful human nuclear transfer, and that future studies could focus on the requirements for genome activation.Terms of Use
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http://nrs.harvard.edu/urn-3:HUL.InstRepos:11315437
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