Identification of the Plasmodium berghei resistance locus 9 linked to survival on chromosome 9
View/ Open
Author
Rodrigo, Evelyn
González-Páez, Gonzalo E
Frazer, Mary
Barnes, S Whitney
Watson, James
Walker, John R
Schmedt, Christian
Winzeler, Elizabeth A
Published Version
https://doi.org/10.1186/1475-2875-12-316Metadata
Show full item recordCitation
Bopp, Selina ER, Evelyn Rodrigo, Gonzalo E González-Páez, Mary Frazer, S Whitney Barnes, Clarissa Valim, James Watson, John R Walker, Christian Schmedt, and Elizabeth A Winzeler. 2013. “Identification of the Plasmodium berghei resistance locus 9 linked to survival on chromosome 9.” Malaria Journal 12 (1): 316. doi:10.1186/1475-2875-12-316. http://dx.doi.org/10.1186/1475-2875-12-316.Abstract
Background: One of the main causes of mortality from severe malaria in Plasmodium falciparum infections is cerebral malaria (CM). An important host genetic component determines the susceptibility of an individual to develop CM or to clear the infection and become semi-immune. As such, the identification of genetic loci associated with susceptibility or resistance may serve to modulate disease severity. Methodology The Plasmodium berghei mouse model for experimental cerebral malaria (ECM) reproduces several disease symptoms seen in human CM, and two different phenotypes, a susceptible (FVB/NJ) and a resistant mouse strain (DBA/2J), were examined. Results: FVB/NJ mice died from infection within ten days, whereas DBA/2J mice showed a gender bias: males survived on average nineteen days and females either died early with signs of ECM or survived for up to three weeks. A comparison of brain pathology between FVB/NJ and DBA/2J showed no major differences with regard to brain haemorrhages or the number of parasites and CD3+ cells in the microvasculature. However, significant differences were found in the peripheral blood of infected mice: For example resistant DBA/2J mice had significantly higher numbers of circulating basophils than did FVB/NJ mice on day seven. Analysis of the F2 offspring from a cross of DBA/2J and FVB/NJ mice mapped the genetic locus of the underlying survival trait to chromosome 9 with a Lod score of 4.9. This locus overlaps with two previously identified resistance loci (char1 and pymr) from a blood stage malaria model. Conclusions: Survival best distinguishes malaria infections between FVB/NJ and DBA/2J mice. The importance of char1 and pymr on chromosome 9 in malaria resistance to P. berghei was confirmed. In addition there was an association of basophil numbers with survival.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3848760/pdf/Terms of Use
This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAACitable link to this page
http://nrs.harvard.edu/urn-3:HUL.InstRepos:11879277
Collections
- SPH Scholarly Articles [6362]
Contact administrator regarding this item (to report mistakes or request changes)