Measuring Markers of Liver Function Using a Micropatterned Paper Device Designed for Blood from a Fingerstick
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Author
Vella, Sarah J.
Beattie, Patrick
Cademartiri, Rebecca
Laromaine, Anna
Martinez, Andres W.
Phillips, Scott T.
Mirica, Katherine A.
Published Version
https://doi.org/10.1021/ac203434xMetadata
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Vella, Sarah J., Patrick Beattie, Rebecca Cademartiri, Anna Laromaine, Andres W. Martinez, Scott T. Phillips, Katherine A. Mirica, and George M. Whitesides. 2012. Measuring Markers of Liver Function Using a Micropatterned Paper Device Designed for Blood from a Fingerstick. Analytical Chemistry 84, no. 6: 2883–2891.Abstract
This paper describes a paper-based microfluidic device that measures two enzymatic markers of liver function (alkaline phosphatase, ALP, and aspartate aminotransferase, AST) and total serum protein. A device consists of four components: (i) a top plastic sheet, (ii) a filter membrane, (iii) a patterned paper chip containing the reagents necessary for analysis, and (iv) a bottom plastic sheet. The device performs both the sample preparation (separating blood plasma from erythrocytes) and the assays; it also enables both qualitative and quantitative analysis of data. The data obtained from the paper-microfluidic devices show standard deviations in calibration runs and “spiked” standards that are acceptable for routine clinical use. This device illustrates a type of test useable for a range of assays in resource-poor settings.Other Sources
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