Classical Flt3L-dependent dendritic cells control immunity to protein vaccine
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Author
Feder, Rachel
Mollah, Shamim
Tse, Sze-Wah
Longhi, Maria Paula
Mehandru, Saurabh
Matos, Ines
Cheong, Cheolho
Ruane, Darren
Brane, Lucas
Teixeira, Angela
Dobrin, Joseph
Mizenina, Olga
Park, Chae Gyu
Meredith, Matthew
Clausen, Björn E.
Nussenzweig, Michel C.
Steinman, Ralph M.
Note: Order does not necessarily reflect citation order of authors.
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https://doi.org/10.1084/jem.20131397Metadata
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Anandasabapathy, N., R. Feder, S. Mollah, S. Tse, M. P. Longhi, S. Mehandru, I. Matos, et al. 2014. “Classical Flt3L-dependent dendritic cells control immunity to protein vaccine.” The Journal of Experimental Medicine 211 (9): 1875-1891. doi:10.1084/jem.20131397. http://dx.doi.org/10.1084/jem.20131397.Abstract
DCs are critical for initiating immunity. The current paradigm in vaccine biology is that DCs migrating from peripheral tissue and classical lymphoid-resident DCs (cDCs) cooperate in the draining LNs to initiate priming and proliferation of T cells. Here, we observe subcutaneous immunity is Fms-like tyrosine kinase 3 ligand (Flt3L) dependent. Flt3L is rapidly secreted after immunization; Flt3 deletion reduces T cell responses by 50%. Flt3L enhances global T cell and humoral immunity as well as both the numbers and antigen capture capacity of migratory DCs (migDCs) and LN-resident cDCs. Surprisingly, however, we find immunity is controlled by cDCs and actively tempered in vivo by migDCs. Deletion of Langerin+ DC or blockade of DC migration improves immunity. Consistent with an immune-regulatory role, transcriptomic analyses reveals different skin migDC subsets in both mouse and human cluster together, and share immune-suppressing gene expression and regulatory pathways. These data reveal that protective immunity to protein vaccines is controlled by Flt3L-dependent, LN-resident cDCs.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4144735/pdf/Terms of Use
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