PPARγ deficiency results in severe, accelerated osteoarthritis associated with aberrant mTOR signalling in the articular cartilage
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Author
Vasheghani, Faezeh
Zhang, Yue
Li, Ying-Hua
Blati, Meryem
Fahmi, Hassan
Lussier, Bertrand
Roughley, Peter
Endisha, Helal
Saffar, Bahareh
Lajeunesse, Daniel
Marshall, Wayne K
Rampersaud, Y Raja
Mahomed, Nizar N
Gandhi, Rajiv
Pelletier, Jean-Pierre
Martel-Pelletier, Johanne
Kapoor, Mohit
Note: Order does not necessarily reflect citation order of authors.
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https://doi.org/10.1136/annrheumdis-2014-205743Metadata
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Vasheghani, F., Y. Zhang, Y. Li, M. Blati, H. Fahmi, B. Lussier, P. Roughley, et al. 2015. “PPARγ deficiency results in severe, accelerated osteoarthritis associated with aberrant mTOR signalling in the articular cartilage.” Annals of the Rheumatic Diseases 74 (3): 569-578. doi:10.1136/annrheumdis-2014-205743. http://dx.doi.org/10.1136/annrheumdis-2014-205743.Abstract
Objectives: We have previously shown that peroxisome proliferator-activated receptor gamma (PPARγ), a transcription factor, is essential for the normal growth and development of cartilage. In the present study, we created inducible cartilage-specific PPARγ knockout (KO) mice and subjected these mice to the destabilisation of medial meniscus (DMM) model of osteoarthritis (OA) to elucidate the specific in vivo role of PPARγ in OA pathophysiology. We further investigated the downstream PPARγ signalling pathway responsible for maintaining cartilage homeostasis. Methods: Inducible cartilage-specific PPARγ KO mice were generated and subjected to DMM model of OA. We also created inducible cartilage-specific PPARγ/mammalian target for rapamycin (mTOR) double KO mice to dissect the PPARγ signalling pathway in OA. Results: Compared with control mice, PPARγ KO mice exhibit accelerated OA phenotype with increased cartilage degradation, chondrocyte apoptosis, and the overproduction of OA inflammatory/catabolic factors associated with the increased expression of mTOR and the suppression of key autophagy markers. In vitro rescue experiments using PPARγ expression vector reduced mTOR expression, increased expression of autophagy markers and reduced the expression of OA inflammatory/catabolic factors, thus reversing the phenotype of PPARγ KO mice chondrocytes. To dissect the in vivo role of mTOR pathway in PPARγ signalling, we created and subjected PPARγ-mTOR double KO mice to the OA model to see if the genetic deletion of mTOR in PPARγ KO mice (double KO) can rescue the accelerated OA phenotype observed in PPARγ KO mice. Indeed, PPARγ-mTOR double KO mice exhibit significant protection/reversal from OA phenotype. Significance PPARγ maintains articular cartilage homeostasis, in part, by regulating mTOR pathway.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4345902/pdf/Terms of Use
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