TRIM28 regulates RNA polymerase II promoter proximal pausing and pause release
View/ Open
Author
Zheng, Xiaofeng
Burkholder, Adam
Motola, Shmulik
Ebmeier, Christopher C.
Levine, Stuart
Fargo, David
Hu, Guang
Taatjes, Dylan J.
Note: Order does not necessarily reflect citation order of authors.
Published Version
https://doi.org/10.1038/nsmb.2878Metadata
Show full item recordCitation
Bunch, H., X. Zheng, A. Burkholder, S. T. Dillon, S. Motola, G. Birrane, C. C. Ebmeier, et al. 2014. “TRIM28 regulates RNA polymerase II promoter proximal pausing and pause release.” Nature structural & molecular biology 21 (10): 876-883. doi:10.1038/nsmb.2878. http://dx.doi.org/10.1038/nsmb.2878.Abstract
Summary Promoter proximal pausing of RNA polymerase II (Pol II) is a major checkpoint in transcription. An unbiased search for novel human proteins that could regulate paused Pol II at the HSPA1B gene identified TRIM28. In vitro analyses indicated HSF1-dependent attenuation of Pol II pausing upon TRIM28 depletion, whereas in vivo data revealed de novo expression of HSPA1B and other known genes regulated by paused Pol II upon TRIM28 knockdown. These results were supported by genome-wide ChIP-sequencing analyses of Pol II occupancy that revealed a global role for TRIM28 in regulating Pol II pausing and pause release. Furthermore, in vivo and in vitro mechanistic studies suggest that transcription-coupled phosphorylation regulates Pol II pause release by TRIM28. Collectively, our findings identify TRIM28 as a novel factor that modulates Pol II pausing and transcriptional elongation at a large number of mammalian genes.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4189995/pdf/Terms of Use
This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAACitable link to this page
http://nrs.harvard.edu/urn-3:HUL.InstRepos:15035062
Collections
- HMS Scholarly Articles [17922]
Contact administrator regarding this item (to report mistakes or request changes)