Now showing items 4922-4941 of 18256

    • An Engineered Calmodulin-Based Allosteric Switch for Peptide Biosensing 

      Meister, Glenna E.; Joshi, Neel S. (Wiley Blackwell (John Wiley & Sons), 2013)
      This work describes the development of a new platform for allosteric protein engineering that takes advantage of the ability of calmodulin to change conformation upon binding to peptide and protein ligands. The switch we ...
    • Engineered catalytic biofilms: Site-specific enzyme immobilization onto E. coli curli nanofibers 

      Botyanszki, Zsofia; Tay, Pei Kun Richie Richie; Nguyen, Peter Q; Nussbaumer, Martin; Joshi, Neel S. (Wiley-Blackwell, 2015)
      Biocatalytic transformations generally rely on purified enzymes or whole cells to perform complex transformations that are used on industrial scale for chemical, drug, and biofuel synthesis, pesticide decontamination, and ...
    • Engineered Hyperactive Integrase for Concerted HIV-1 DNA Integration 

      Li, Min; Jurado, Kellie A.; Lin, Shiqiang; Engelman, Alan; Craigie, Robert (Public Library of Science, 2014)
      The DNA cutting and joining reactions of HIV-1 integration are catalyzed by integrase (IN), a viral protein that functions as a tetramer bridging the two viral DNA ends (intasome). Two major obstacles for biochemical and ...
    • Engineered Living Materials: Engineered Living Materials: Prospects and Challenges for Using Biological Systems to Direct the Assembly of Smart Materials 

      Nguyen, Peter; Courchesne, Noémie-Manuelle Dorval; Duraj-Thatte, Anna; Praveschotinunt, Pichet; Joshi, Neel (Wiley, 2018-05)
      Vast potential exists for the development of novel, engineered platforms that manipulate biology for the production of programmed advanced materials. Such systems would possess the autonomous, adaptive, and self‐healing ...
    • Engineered Myosins Drive Filopodial Transport 

      Erdogan, Burcu; Whited, Jessica L. (Springer Science and Business Media LLC, 2021-02-01)
      Engineered, light-inducible artificial myosin motors enable selective and direct manipulation of filopodial extensions and provide refined tools to control intracellular cargo transport in vivo.
    • Engineered pegRNAs improve prime editing efficiency 

      Nelson, James W.; Randolph, Peyton B.; Shen, Simon; Everette, Kelcee A.; Chen, Peter; Anzalone, Andrew; An, Meirui; Newby, Gregory; Chen, Jonathan; Hsu, Alvin; Liu, David (Springer Science and Business Media LLC, 2021-10-04)
      Prime editing enables the installation of virtually any combination of point mutations, small insertions or small deletions in the DNA of living cells. A prime editing guide RNA (pegRNA) directs the prime editor protein ...
    • Engineering acyl carrier protein to enhance production of shortened fatty acids 

      Liu, Xueliang; Hicks, Wade M.; Silver, Pamela A.; Way, Jeffrey C. (BioMed Central, 2016)
      Background: The acyl carrier protein (ACP) is an essential and ubiquitous component of microbial synthesis of fatty acids, the natural precursor to biofuels. Natural fatty acids usually contain long chains of 16 or more ...
    • Engineering and Identifying Supercharged Proteins for Macromolecule Delivery Into Mammalian Cells 

      Thompson, David B.; Cronican, James; Liu, David Ruchien (Elsevier BV, 2012)
      Supercharged proteins are a class of engineered or naturally occurring proteins with unusually high net positive or negative theoretical charge. Both supernegatively and superpositively charged proteins exhibit a remarkable ...
    • Engineering Directed Excitonic Energy Transfer 

      Perdomo, Alejandro; Vogt, Leslie; Najmaie, Ali; Aspuru-Guzik, Alan (American Institute of Physics, 2010)
      We provide an intuitive platform for engineering exciton transfer dynamics. We show that careful consideration of the spectral density, which describes the system-bath interaction, leads to opportunities to engineer exciton ...
    • Engineering Escherichia coli into a Protein Delivery System for Mammalian Cells 

      Reeves, Analise Z.; Spears, William E.; Du, Juan; Tan, Kah Yong; Wagers, Amy J.; Lesser, Cammie F. (American Chemical Society, 2015)
      Many Gram-negative pathogens encode type 3 secretion systems, sophisticated nanomachines that deliver proteins directly into the cytoplasm of mammalian cells. These systems present attractive opportunities for therapeutic ...
    • Engineering Genetically-Encoded Mineralization and Magnetism via Directed Evolution 

      Liu, Xueliang; Lopez, Paola A.; Giessen, Tobias W.; Giles, Michael; Way, Jeffrey C.; Silver, Pamela A. (Nature Publishing Group, 2016)
      Genetically encoding the synthesis of functional nanomaterials such as magnetic nanoparticles enables sensitive and non-invasive biological sensing and control. Via directed evolution of the natural iron-sequestering ...
    • Engineering microdeletions and microduplications by targeting segmental duplications with CRISPR 

      Tai, Derek J. C.; Ragavendran, Ashok; Manavalan, Poornima; Stortchevoi, Alexei; Seabra, Catarina M.; Erdin, Serkan; Collins, Ryan L.; Blumenthal, Ian; Chen, Xiaoli; Shen, Yiping; Sahin, Mustafa; Zhang, Chengsheng; Lee, Charles; Gusella, James F.; Talkowski, Michael E. (2016)
      Recurrent, reciprocal genomic disorders resulting from non-allelic homologous recombination (NAHR) between near-identical segmental duplications (SDs) are a major cause of human disease, often producing phenotypically ...
    • Engineering Shadows to Fabricate Optical Metasurfaces 

      Nemiroski, Alex; Gonidec, Mathieu; Fox, Jerome Michael; Jean-Remy, Philip; Turnage, Evan; Whitesides, George McClelland (American Chemical Society (ACS), 2014)
      Optical metasurfaces—patterned arrays of plasmonic nanoantennas that enable the precise manipulation of light–matter interactions—are emerging as critical components in many nanophotonic materials, including planar ...
    • Engineering superfluidity in Bose-Fermi mixtures of ultracold atoms 

      Wang, D.-W.; Lukin, Mikhail D.; Demler, Eugene A. (American Physical Society (APS), 2005)
      We investigate many-body phase diagrams of atomic boson-fermion mixtures loaded in the two-dimensional optical lattice. Bosons mediate an attractive, finite-range interaction between fermions, leading to fermion pairing ...
    • Engineering the control of mosquito-borne infectious diseases 

      Gabrieli, Paolo; Smidler, Andrea; Catteruccia, Flaminia (BioMed Central, 2014)
      Recent advances in genetic engineering are bringing new promise for controlling mosquito populations that transmit deadly pathogens. Here we discuss past and current efforts to engineer mosquito strains that are refractory ...
    • English Reflexive Logophors 

      Charnavel, Isabelle Carole; Zlogar, Christina Diane (2015)
    • English-language Studies of Precious Scrolls: a Bibliographical Survey 

      Idema, Wilt Lukas (Maney Publishing, 2012)
    • Enhanced aerosol particle growth sustained by high continental chlorine emission in India 

      Gunthe, Sachin S.; Liu, Pengfei; Panda, Upasana; Raj, Subha S.; Sharma, Amit; Darbyshire, Eoghan; Reyes-Villegas, Ernesto; Allan, James; Chen, Ying; Wang, Xuan; Song, Shaojie; Pöhlker, Mira L.; Shi, Liuhua; Wang, Yu; Kommula, Snehitha M.; Liu, Tianjia; Ravikrishna, R.; McFiggans, Gordon; Mickley, Loretta; Martin, Scot; Pöschl, Ulrich; Andreae, Meinrat O.; Coe, Hugh; Coe (Springer Science and Business Media LLC, 2021-01-25)
      Many cities in India experience severe deterioration of air quality in winter. Particulate matter is a key atmospheric pollutant that impacts millions of people. In particular, high levels of particulate matter reduce ...
    • Enhanced Antiferromagnetic Exchange between Magnetic Impurities in a Superconducting Host 

      Yao, Norman Ying; Glazman, Leonid I; Demler, Eugene A.; Lukin, Mikhail D.; Sau, Jay D. (American Physical Society (APS), 2014)
      It is generally believed that superconductivity only weakly affects the indirect exchange between magnetic impurities. If the distance r between impurities is smaller than than the superconducting coherence length \((r ...