A comparison of non-integrating reprogramming methods
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Author
Brickler, Thomas R
Entwisle, Samuel
Chan, Karrie
Cianci, Amelia
DeVine, Alexander
Ettenger, Andrew
Fitzgerald, Kelly
Godfrey, Michelle
Gupta, Dipti
McPherson, Jade
Malwadkar, Prerana
Gupta, Manav
Bell, Blair
Doi, Akiko
Jung, Namyoung
Li, Xin
Brookes, Emily
Cherry, Anne B C
Demirbas, Didem
Feinberg, Andrew P
Note: Order does not necessarily reflect citation order of authors.
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https://doi.org/10.1038/nbt.3070Metadata
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Schlaeger, Thorsten M, Laurence Daheron, Thomas R Brickler, Samuel Entwisle, Karrie Chan, Amelia Cianci, Alexander DeVine, et al. 2014. “A Comparison of Non-Integrating Reprogramming Methods.” Nature Biotechnology 33 (1) (December 1): 58–63. doi:10.1038/nbt.3070.Abstract
Human induced pluripotent stem cells (hiPSCs1, 2, 3) are useful in disease modeling and drug discovery, and they promise to provide a new generation of cell-based therapeutics. To date there has been no systematic evaluation of the most widely used techniques for generating integration-free hiPSCs. Here we compare Sendai-viral (SeV)4, episomal (Epi)5 and mRNA transfection mRNA6 methods using a number of criteria. All methods generated high-quality hiPSCs, but significant differences existed in aneuploidy rates, reprogramming efficiency, reliability and workload. We discuss the advantages and shortcomings of each approach, and present and review the results of a survey of a large number of human reprogramming laboratories on their independent experiences and preferences. Our analysis provides a valuable resource to inform the use of specific reprogramming methods for different laboratories and different applications, including clinical translation.Terms of Use
This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAACitable link to this page
http://nrs.harvard.edu/urn-3:HUL.InstRepos:22556353
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