Coverage recommendations for methylation analysis by whole genome bisulfite sequencing
Citation
Ziller, Michael J., Kasper D. Hansen, Alexander Meissner, and Martin J. Aryee. 2014. “Coverage recommendations for methylation analysis by whole genome bisulfite sequencing.” Nature methods 12 (3): 230-232. doi:10.1038/nmeth.3152. http://dx.doi.org/10.1038/nmeth.3152.Abstract
Whole genome bisulfite sequencing (WGBS) allows genome-wide DNA methylation profiling but the associated high sequencing costs continue to limit its widespread application. We utilized several high coverage reference data sets to experimentally determine minimal sequencing requirements. Here, we present data derived recommendations for minimum sequencing depth for WGBS libraries, highlight what is gained with increasing coverage and discuss the trade off between sequencing depth and number of assayed replicates.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4344394/pdf/Terms of Use
This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAACitable link to this page
http://nrs.harvard.edu/urn-3:HUL.InstRepos:22856906
Collections
- FAS Scholarly Articles [18292]
- HMS Scholarly Articles [17922]
- SPH Scholarly Articles [6362]
Contact administrator regarding this item (to report mistakes or request changes)