All β Cells Contribute Equally to Islet Growth and Maintenance

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All β Cells Contribute Equally to Islet Growth and Maintenance

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Title: All β Cells Contribute Equally to Islet Growth and Maintenance
Author: Brennand, Kristen; Melton, Douglas; Huangfu, Danwei

Note: Order does not necessarily reflect citation order of authors.

Citation: Brennand, Kristen, Danwei Huangfu and Doug Melton. 2007. All β cells contribute equally to islet growth and maintenance. PLoS Biology 5(7): e163.
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Abstract: In healthy adult mice, the β cell population is not maintained by stem cells but instead by the replication of differentiated β cells. It is not known, however, whether all cells contribute equally to growth and maintenance, as it may be that some cells replicate while others do not. Understanding precisely which cells are responsible for β cell replication will inform attempts to expand β cells in vitro, a potential source for cell replacement therapy to treat diabetes. Two experiments were performed to address this issue. First, the level of fluorescence generated by a pulse of histone 2B–green fluorescent protein (H2BGFP) expression was followed over time to determine how this marker is diluted with cell division; a uniform loss of label across the entire β cell population was observed. Second, clonal analysis of dividing β cells was completed; all clones were of comparable size. These results support the conclusion that the β cell pool is homogeneous with respect to replicative capacity and suggest that all β cells are candidates for in vitro expansion. Given similar observations in the hepatocyte population, we speculate that for tissues lacking an adult stem cell, they are replenished equally by replication of all differentiated cells.
Published Version: http://dx.doi.org/10.1371/journal.pbio.0050163
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:2640563

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  • FAS Scholarly Articles [7594]
    Peer reviewed scholarly articles from the Faculty of Arts and Sciences of Harvard University
 
 

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