Virus-induced Gene Silencing as a Tool for Functional Analyses in the Emerging Model Plant Aquilegia (Coumbine, Ranunculaceae)

DSpace/Manakin Repository

Virus-induced Gene Silencing as a Tool for Functional Analyses in the Emerging Model Plant Aquilegia (Coumbine, Ranunculaceae)

Citable link to this page

. . . . . .

Title: Virus-induced Gene Silencing as a Tool for Functional Analyses in the Emerging Model Plant Aquilegia (Coumbine, Ranunculaceae)
Author: Gould, Billie; Kramer, Elena

Note: Order does not necessarily reflect citation order of authors.

Citation: Gould, Billie and Elena M. Kramer. 2007. Virus-induced gene silencing as a tool for functional analyses in the emerging model plant Aquilegia (columbine, Ranunculaceae). Plant Methods 3(6): 1-12.
Full Text & Related Files:
Abstract: Background: The lower eudicot genus Aquilegia, commonly known as columbine, is currently the subject of extensive genetic and genomic research aimed at developing this taxon as a new model for the study of ecology and evolution. The ability to perform functional genetic analyses is a critical component of this development process and ultimately has the potential to provide insight into the genetic basis for the evolution of a wide array of traits that differentiate flowering plants. Aquilegia is of particular interest due to both its recent evolutionary history, which involves a rapid adaptive radiation, and its intermediate phylogenetic position between core eudicot (e. g., Arabidopsis) and grass (e. g., Oryza) model species. Results: Here we demonstrate the effective use of a reverse genetic technique, virus-induced gene silencing (VIGS), to study gene function in this emerging model plant. Using Agrobacterium mediated transfer of tobacco rattle virus (TRV) based vectors, we induce silencing of PHYTOENE DESATURASE (AqPDS) in Aquilegia vulgaris seedlings, and ANTHOCYANIDIN SYNTHASE (AqANS) and the B-class floral organ identity gene PISTILLATA in A. vulgaris flowers. For all of these genes, silencing phenotypes are associated with consistent reduction in endogenous transcript levels. In addition, we show that silencing of AqANS has no effect on overall floral morphology and is therefore a suitable marker for the identification of silenced flowers in dual-locus silencing experiments. Conclusion: Our results show that TRV-VIGS in Aquilegia vulgaris allows data to be rapidly obtained and can be reproduced with effective survival and silencing rates. Furthermore, this method can successfully be used to evaluate the function of early-acting developmental genes. In the future, data derived from VIGS analyses will be combined with large-scale sequencing and microarray experiments already underway in order to address both recent and ancient evolutionary questions.
Published Version: http://www.plantmethods.com/content/3/1/6
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:2641740

Show full Dublin Core record

This item appears in the following Collection(s)

  • FAS Scholarly Articles [7374]
    Peer reviewed scholarly articles from the Faculty of Arts and Sciences of Harvard University
 
 

Search DASH


Advanced Search
 
 

Submitters