Direct haplotyping of kilobase-size DNA using carbon nanotube probes

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Direct haplotyping of kilobase-size DNA using carbon nanotube probes

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Title: Direct haplotyping of kilobase-size DNA using carbon nanotube probes
Author: Cheung, Chin Li; Housman, David E.; Woolley, Adam T.; Guillemette, Chantal; Lieber, Charles

Note: Order does not necessarily reflect citation order of authors.

Citation: Woolley, Adam T., Chantal Guillemette, Chin Li Cheung, David E. Housman, and Charles M. Lieber. 2000. Direct haplotyping of kilobase-size DNA using carbon nanotube probes. Nature Biotechnology 18(7): 760-763.
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Abstract: We have implemented a method for multiplexed detection of polymorphic sites and direct determination of haplotypes in 10-kilobase- size DNA fragments using single-walled carbon nanotube (SWNT) atomic force microscopy (AFM) probes. Labeled oligonucleotides are hybridized specifically to complementary target sequences in template DNA, and the positions of the tagged sequences are detected by direct SWNT tip imaging. We demonstrated this concept by detecting streptavidin and IRD800 labels at two different sequences in M13mp18. Our approach also permits haplotype determination from simple visual inspection of AFM images of individual DNA molecules, which we have done on UGT1A7, a gene under study as a cancer risk factor. The haplotypes of individuals heterozygous at two critical loci, which together influence cancer risk, can be easily and directly distinguished from AFM images. The application ofnique to haplotyping in population-based genetic disease studies and other genomic screening problems is discussed.
Published Version: http://dx.doi.org/10.1038/77760
Other Sources: http://digitalcommons.unl.edu/chemistrycheung/7/
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:2710489

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  • FAS Scholarly Articles [7078]
    Peer reviewed scholarly articles from the Faculty of Arts and Sciences of Harvard University
 
 

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