Show simple item record

dc.contributor.authorBrandenburg, Boerries
dc.contributor.authorLee, Lily Y.
dc.contributor.authorLakadamyali, Melike
dc.contributor.authorRust, Michael J.
dc.contributor.authorZhuang, Xiaowei
dc.contributor.authorHogle, James M.
dc.date.accessioned2009-03-24T20:52:42Z
dc.date.issued2007
dc.identifier.citationBrandenburg Boerries, Lily Y. Lee, Melike Lakadamyali, Michael J. Rust, Xiaowei Zhuang, and James M. Hogle. 2007. Imaging poliovirus entry in live cells. PLoS Biology 5(7): e183. doi:10.1371/journal.pbio.0050183en
dc.identifier.issn1545-7885en
dc.identifier.issn1544-9173en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:2710572
dc.description.abstractViruses initiate infection by transferring their genetic material across a cellular membrane and into the appropriate compartment of the cell. The mechanisms by which animal viruses, especially nonenveloped viruses, deliver their genomes are only poorly understood. This is due in part to technical difficulties involved in direct visualization of viral gene delivery and to uncertainties in distinguishing productive and nonproductive pathways caused by the high particle-to–plaque forming unit ratio of most animal viruses. Here, we combine an imaging assay that simultaneously tracks the viral capsid and genome in live cells with an infectivity-based assay for RNA release to characterize the early events in the poliovirus (PV) infection. Effects on RNA genome delivery from inhibitors of cell trafficking pathways were probed systematically by both methods. Surprisingly, we observe that genome release by PV is highly efficient and rapid, and thus does not limit the overall infectivity or the infection rate. The results define a pathway in which PV binds to receptors on the cell surface and enters the cell by a clathrin-, caveolin-, flotillin-, and microtubule-independent, but tyrosine kinase- and actin-dependent, endocytic mechanism. Immediately after the internalization of the virus particle, genome release takes place from vesicles or tightly sealed membrane invaginations located within 100–200 nm of the plasma membrane. These results settle a long-lasting debate of whether PV directly breaks the plasma membrane barrier or relies on endocytosis to deliver its genome into the cell. We expect this imaging assay to be broadly applicable to the investigation of entry mechanisms for nonenveloped viruses.en
dc.description.sponsorshipChemistry and Chemical Biologyen
dc.description.sponsorshipPhysicsen
dc.description.sponsorshipMolecular and Cellular Biology
dc.language.isoen_USen
dc.publisherPublic Library of Scienceen
dc.relation.isversionofhttp://dx.doi.org/10.1371/journal.pbio.0050183en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC1914398/pdf/pbio.0050183.pdf
dash.licenseLAA
dc.subjectchemical biologyen
dc.subjectinfectious diseasesen
dc.subjectcell biologyen
dc.subjectvirologyen
dc.subjectmicrobiologyen
dc.titleImaging Poliovirus Entry in Live Cellsen
dc.relation.journalPLoS Biologyen
dash.depositing.authorZhuang, Xiaowei
dc.identifier.doi10.1371/journal.pbio.0050183*
dash.contributor.affiliatedZhuang, Xiaowei


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record