Identification of Eukaryotic Promoter Regulatory Elements Using Nonhomologous Random Recombination

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Identification of Eukaryotic Promoter Regulatory Elements Using Nonhomologous Random Recombination

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Title: Identification of Eukaryotic Promoter Regulatory Elements Using Nonhomologous Random Recombination
Author: Liu, David; Doyon, Jeffrey B.

Note: Order does not necessarily reflect citation order of authors.

Citation: Doyon, Jeffrey R., and David R. Liu. 2007. Identification of eukaryotic promoter regulatory elements using nonhomologous random recombination. Nucleic Acids Research 35(17): 5851-5860.
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Abstract: Understanding the regulatory logic of a eukaryotic promoter requires the elucidation of the regulatory elements within that promoter. Current experimental or computational methods to discover regulatory motifs within a promoter can be labor intensive and may miss redundant, unprecedented or weakly activating elements. We have developed an unbiased combinatorial approach to rapidly identify new upstream activating sequences (UASs) in a promoter. This approach couples nonhomologous random recombination with an in vivo screen to efficiently identify UASs and does not rely on preconceived hypotheses about promoter regulation or on similarity to known activating sequences. We validated this method using the unfolded protein response (UPR) in yeast and were able to identify both known and potentially novel UASs involved in the UPR. One of the new UASs discovered using this approach implicates Crz1 as a possible activator of Hac1, a transcription factor involved in the UPR. This method has several advantages over existing methods for UAS discovery including its speed, potential generality, sensitivity and lack of false positives and negatives.
Published Version: http://dx.doi.org/10.1093/nar/gkm634
Other Sources: http://evolve.harvard.edu/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:2958223

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  • FAS Scholarly Articles [7362]
    Peer reviewed scholarly articles from the Faculty of Arts and Sciences of Harvard University
 
 

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