Identification of Eukaryotic Promoter Regulatory Elements Using Nonhomologous Random Recombination

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Identification of Eukaryotic Promoter Regulatory Elements Using Nonhomologous Random Recombination

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dc.contributor.author Liu, David
dc.contributor.author Doyon, Jeffrey B.
dc.date.accessioned 2009-05-14T18:24:16Z
dc.date.issued 2007
dc.identifier.citation Doyon, Jeffrey R., and David R. Liu. 2007. Identification of eukaryotic promoter regulatory elements using nonhomologous random recombination. Nucleic Acids Research 35(17): 5851-5860. en
dc.identifier.issn 0305-1048 en
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:2958223
dc.description.abstract Understanding the regulatory logic of a eukaryotic promoter requires the elucidation of the regulatory elements within that promoter. Current experimental or computational methods to discover regulatory motifs within a promoter can be labor intensive and may miss redundant, unprecedented or weakly activating elements. We have developed an unbiased combinatorial approach to rapidly identify new upstream activating sequences (UASs) in a promoter. This approach couples nonhomologous random recombination with an <i>in vivo</i> screen to efficiently identify UASs and does not rely on preconceived hypotheses about promoter regulation or on similarity to known activating sequences. We validated this method using the unfolded protein response (UPR) in yeast and were able to identify both known and potentially novel UASs involved in the UPR. One of the new UASs discovered using this approach implicates Crz1 as a possible activator of Hac1, a transcription factor involved in the UPR. This method has several advantages over existing methods for UAS discovery including its speed, potential generality, sensitivity and lack of false positives and negatives. en
dc.description.sponsorship Chemistry and Chemical Biology en
dc.language.iso en_US en
dc.publisher Oxford University Press en
dc.relation.isversionof http://dx.doi.org/10.1093/nar/gkm634 en
dc.relation.hasversion http://evolve.harvard.edu/ en
dash.license LAA
dc.title Identification of Eukaryotic Promoter Regulatory Elements Using Nonhomologous Random Recombination en
dc.relation.journal Nucleic Acids Research en
dash.depositing.author Liu, David

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  • FAS Scholarly Articles [7594]
    Peer reviewed scholarly articles from the Faculty of Arts and Sciences of Harvard University

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