Protein Arms in the Kinetochore-Microtubule Interface of the Yeast DASH Complex

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Protein Arms in the Kinetochore-Microtubule Interface of the Yeast DASH Complex

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Title: Protein Arms in the Kinetochore-Microtubule Interface of the Yeast DASH Complex
Author: Harrison, Stephen; King, David S.; Miranda, JJ L.

Note: Order does not necessarily reflect citation order of authors.

Citation: Miranda, JJ L., David S. King, and Stephen C. Harrison. 2007. Protein arms in the kinetochore-microtubule interface of the yeast DASH complex. Molecular Biology of the Cell 18, no. 7: 2503-2510.
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Abstract: The yeast DASH complex is a heterodecameric component of the kinetochore necessary for accurate chromosome segregation. DASH forms closed rings around microtubules with a large gap between the DASH ring and the microtubule cylinder. We characterized the microtubule-binding properties of limited proteolysis products and subcomplexes of DASH, thus identifying candidate polypeptide extensions involved in establishing the DASH-microtubule interface. The acidic C-terminal extensions of tubulin subunits are not essential for DASH binding. We also measured the molecular mass of DASH rings on microtubules with scanning transmission electron microscopy and found that approximately 25 DASH heterodecamers assemble to form each ring. Dynamic association and relocation of multiple flexible appendages of DASH may allow the kinetochore to translate along the microtubule surface.
Published Version: http://dx.doi.org/10.1091/mbc.E07-02-0135
Other Sources: http://crystal.harvard.edu/PDFs/dash_arms.pdf
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:3109301

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  • FAS Scholarly Articles [7374]
    Peer reviewed scholarly articles from the Faculty of Arts and Sciences of Harvard University
 
 

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