Characterization of Individual Polynucleotide Molecules Using a Membrane Channel

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Characterization of Individual Polynucleotide Molecules Using a Membrane Channel

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Title: Characterization of Individual Polynucleotide Molecules Using a Membrane Channel
Author: Branton, Daniel; Kasianowicz, John J.; Deamer, David W.; Brandin, Eric

Note: Order does not necessarily reflect citation order of authors.

Citation: Kasianowicz, John J., Eric Brandin, Daniel Branton, and David W. Deamer. 1996. Characterization of individual polynucleotide molecules using a membrane channel. Proceedings of the National Academy of Sciences 93(24): 13770-13773.
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Abstract: We show that an electric field can drive single-stranded RNA and DNA molecules through a 2.6-nm diameter ion channel in a lipid bilayer membrane. Because the channel diameter can accommodate only a single strand of RNA or DNA, each polymer traverses the membrane as an extended chain that partially blocks the channel. The passage of each molecule is detected as a transient decrease of ionic current whose duration is proportional to polymer length. Channel blockades can therefore be used to measure polynucleotide length. With further improvements, the method could in principle provide direct, high-speed detection of the sequence of bases in single molecules of DNA or RNA.
Published Version: http://dx.doi.org/10.1073/pnas.93.24.13770
Other Sources: http://www.mcb.harvard.edu/branton/kasianowicz01.pdf
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:3109362

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  • FAS Scholarly Articles [7220]
    Peer reviewed scholarly articles from the Faculty of Arts and Sciences of Harvard University
 
 

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