Unzipping Kinetics of Double-Stranded DNA in a Nanopore

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Unzipping Kinetics of Double-Stranded DNA in a Nanopore

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Title: Unzipping Kinetics of Double-Stranded DNA in a Nanopore
Author: Branton, Daniel; Lubensky, David K.; Nyamwanda, Jacqueline A.; Sauer-Budge, Alexis F.

Note: Order does not necessarily reflect citation order of authors.

Citation: Sauer-Budge, Alexis F., Jacqueline A. Nyamwanda, David K. Lubensky, and Daniel Branton. 2003. Unzipping kinetics of double-stranded DNA in a nanopore. Physical Review Letters 90(23): 238101.
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Abstract: We studied the unzipping of single molecules of double-stranded DNA by pulling one of their two strands through a narrow protein pore. Polymerase chain reaction analysis yielded the first direct proof of DNA unzipping in such a system. The time to unzip each molecule was inferred from the ionic current signature of DNA traversal. The distribution of times to unzip under various experimental conditions fit a simple kinetic model. Using this model, we estimated the enthalpy barriers to unzipping and the effective charge of a nucleotide in the pore, which was considerably smaller than previously assumed.
Published Version: http://dx.doi.org/10.1103/PhysRevLett.90.238101
Other Sources: http://www.mcb.harvard.edu/branton/Sauer-BudgePhysicalReviewLetters.pdf
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:3109364

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  • FAS Scholarly Articles [6464]
    Peer reviewed scholarly articles from the Faculty of Arts and Sciences of Harvard University
 
 

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