Multiple Formaldehyde Oxidation/Detoxification Pathways in Burkholderia Fungorum LB400

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Multiple Formaldehyde Oxidation/Detoxification Pathways in Burkholderia Fungorum LB400

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Title: Multiple Formaldehyde Oxidation/Detoxification Pathways in Burkholderia Fungorum LB400
Author: Marx, Christopher; Lidstrom, Mary E.; Chistoserdova, Ludmila; Miller, Jonathan A.

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Citation: Marx, Christopher J., Jonathan A. Miller, Ludmila Chistoserdova, and Mary E. Lidstrom. 2004. Multiple formaldehyde oxidation/detoxification pathways in Burkholderia fungorum LB400. Journal of Bacteriology 186, no. 7: 2173-2178.
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Abstract: Burkholderia species are free-living bacteria with a versatile metabolic lifestyle. The genome of B. fungorum LB400 is predicted to encode three different pathways for formaldehyde oxidation: an NAD-linked, glutathione (GSH) -independent formaldehyde dehydrogenase; an NAD-linked, GSH-dependent formaldehyde oxidation system; and a tetrahydromethanopterin-methanofuran-dependent formaldehyde oxidation system. The other Burkholderia species for which genome sequences are available, B. mallei, B. pseudomallei, and B. cepacia, are predicted to contain only the first two of these pathways. The roles of the three putative formaldehyde oxidation pathways in B.fungorum LB400 have been assessed via knockout mutations in each of these pathways, as well as in all combinations of knockouts. The resulting mutants have the expected loss of enzyme activities and exhibit defects of varying degrees of severity during growth on choline, a formaldehyde-producing substrate. Our data suggest that all three pathways are involved in formaldehyde detoxification and are functionally redundant under the tested conditions.
Published Version: http://dx.doi.org/10.1128/JB.186.7.2173-2178.2004
Other Sources: http://www.oeb.harvard.edu/faculty/marx/marxlabFrameset.htm
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:3137805

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  • FAS Scholarly Articles [7374]
    Peer reviewed scholarly articles from the Faculty of Arts and Sciences of Harvard University
 
 

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