Vasa Protein Expression is Restricted to the Small Micromeres of the Sea Urchin, but is Inducible in Other Lineages Early in Development

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Vasa Protein Expression is Restricted to the Small Micromeres of the Sea Urchin, but is Inducible in Other Lineages Early in Development

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dc.contributor.author Wessel, Gary
dc.contributor.author McClay, David
dc.contributor.author Oliveri, Paola
dc.contributor.author George, Sophie
dc.contributor.author Extavour, Cassandra
dc.contributor.author Song, Jia L.
dc.contributor.author Gustafson, Eric
dc.contributor.author Juliano, Celina E.
dc.contributor.author Lopez, Manuel
dc.contributor.author Voronina, Ekaterina
dc.date.accessioned 2009-07-09T19:58:08Z
dc.date.issued 2008
dc.identifier.citation Voronina, Ekaterina, Manuel Lopez, Celina E. Juliano, Eric Gustafson, Jia L. Song, Cassandra Extavour, Sophie Georgeet al. 2008. Vasa protein expression is restricted to the small micromeres of the sea urchin, but is inducible in other lineages early in development. Developmental Biology 314, no. 2: 276-286. en
dc.identifier.issn 0012-1606 en
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:3160487
dc.description.abstract Vasa is a DEAD-box RNA helicase that functions in translational regulation of specific mRNAs. In many animals it is essential for germ line development and may have a more general stem cell role. Here we identify vasa in two sea urchin species and analyze the regulation of its expression. We find that vasa protein accumulates in only a subset of cells containing vasa mRNA. In contrast to vasa mRNA, which is present uniformly throughout all cells of the early embryo, vasa protein accumulates selectively in the 16-cell stage micromeres, and then is restricted to the small micromeres through gastrulation to larval development. Manipulating early embryonic fate specification by blastomere separations, exposure to lithium, and dominant-negative cadherin each suggest that, although vasa protein accumulation in the small micromeres is fixed, accumulation in other cells of the embryo is inducible. Indeed, we find that embryos in which micromeres are removed respond by significant up-regulation of vasa protein translation, followed by spatial restriction of the protein late in gastrulation. Overall, these results support the contention that sea urchins do not have obligate primordial germ cells determined in early development, that vasa may function in an early stem cell population of the embryo, and that vasa expression in this embryo is restricted early by translational regulation to the small micromere lineage. en
dc.description.sponsorship Organismic and Evolutionary Biology en
dc.language.iso en_US en
dc.publisher Elsevier en
dc.relation.isversionof http://dx.doi.org/10.1016/j.ydbio.2007.11.039 en
dash.license LAA
dc.subject small micromeres en
dc.subject sea urchin en
dc.subject vasa en
dc.title Vasa Protein Expression is Restricted to the Small Micromeres of the Sea Urchin, but is Inducible in Other Lineages Early in Development en
dc.relation.journal Developmental Biology en
dash.depositing.author Extavour, Cassandra

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  • FAS Scholarly Articles [7106]
    Peer reviewed scholarly articles from the Faculty of Arts and Sciences of Harvard University

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