Conversion of 5-Methylcytosine to 5-Hydroxymethylcytosine in Mammalian DNA by the MLL Partner TET1

DSpace/Manakin Repository

Conversion of 5-Methylcytosine to 5-Hydroxymethylcytosine in Mammalian DNA by the MLL Partner TET1

Show simple item record

dc.contributor.author Tahiliani, Mamta V.
dc.contributor.author Koh, Kian Peng
dc.contributor.author Shen, Yinghua
dc.contributor.author Pastor, William Abraham
dc.contributor.author Bandukwala, Hozefa S
dc.contributor.author Brudno, Yevgeny
dc.contributor.author Agarwal, Suneet
dc.contributor.author Iyer, Lakshminarayan M.
dc.contributor.author Liu, David Ruchien
dc.contributor.author Aravind, L
dc.contributor.author Rao, Anjana
dc.date.accessioned 2009-11-20T19:52:06Z
dc.date.issued 2009
dc.identifier.citation Tahiliani, Mamta, Kian Peng Koh, Yinghua Shen, William A. Pastor, Hozefa Bandukwala, Yevgeny Brudno, Suneet Agarwal, Lakshminarayan M. Iyer, David R. Liu, L. Aravind, and Anjana Rao. 2009. Conversion of 5-methylcytosine to 5-hydroxymethylcytosine in mammalian DNA by MLL partner TET1. Science 324(5929): 930-935. en_US
dc.identifier.issn 0193-4511 en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:3415331
dc.description.abstract DNA cytosine methylation is crucial for retrotransposon silencing and mammalian development. In a computational search for enzymes that could modify 5-methylcytosine (5mC), we identified TET proteins as mammalian homologs of the trypanosome proteins JBP1 and JBP2, which have been proposed to oxidize the 5-methyl group of thymine. We show here that TET1, a fusion partner of the MLL gene in acute myeloid leukemia, is a 2-oxoglutarate (2OG)- and Fe(II)-dependent enzyme that catalyzes conversion of 5mC to 5-hydroxymethylcytosine (hmC) in cultured cells and in vitro. hmC is present in the genome of mouse embryonic stem cells, and hmC levels decrease upon RNA interference–mediated depletion of TET1. Thus, TET proteins have potential roles in epigenetic regulation through modification of 5mC to hmC. en_US
dc.description.sponsorship Chemistry and Chemical Biology en_US
dc.language.iso en_US en_US
dc.publisher American Association for the Advancement of Science en_US
dc.relation.isversionof http://dx.doi.org/10.1126/science.1170116 en_US
dc.relation.hasversion http://evolve.harvard.edu/55-hmC-Science.pdf en_US
dash.license OAP
dc.title Conversion of 5-Methylcytosine to 5-Hydroxymethylcytosine in Mammalian DNA by the MLL Partner TET1 en_US
dc.type Journal Article en_US
dc.description.version Accepted Manuscript en_US
dc.relation.journal Science en_US
dash.depositing.author Liu, David Ruchien
dc.date.available 2009-11-20T19:52:06Z

Files in this item

Files Size Format View
Tahiliani_Conversion5methylcytosine.pdf 2.614Mb PDF View/Open

This item appears in the following Collection(s)

  • FAS Scholarly Articles [7587]
    Peer reviewed scholarly articles from the Faculty of Arts and Sciences of Harvard University

Show simple item record

 
 

Search DASH


Advanced Search
 
 

Submitters