A Stochastic Single-Molecule Event Triggers Phenotype Switching of a Bacterial Cell

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A Stochastic Single-Molecule Event Triggers Phenotype Switching of a Bacterial Cell

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Title: A Stochastic Single-Molecule Event Triggers Phenotype Switching of a Bacterial Cell
Author: Choi, Paul Jongjoon; Cai, Long; Frieda, Kirsten; Xie, Xiaoliang Sunney

Note: Order does not necessarily reflect citation order of authors.

Citation: Choi, Paul J., Long Cai, Kirsten Frieda, and Xiaoliang Sunney Xie. 2008. A stochastic single-molecule event triggers phenotype switching of a bacterial cell. Science 322(5900): 442-446.
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Abstract: By monitoring fluorescently labeled lactose permease with single-molecule sensitivity, we investigated the molecular mechanism of how an Escherichia coli cell with the lac operon switches from one phenotype to another. At intermediate inducer concentrations, a population of genetically identical cells exhibits two phenotypes: induced cells with highly fluorescent membranes and uninduced cells with a small number of membrane-bound permeases. We found that this basal-level expression results from partial dissociation of the tetrameric lactose repressor from one of its operators on looped DNA. In contrast, infrequent events of complete dissociation of the repressor from DNA result in large bursts of permease expression that trigger induction of the lac operon. Hence, a stochastic single-molecule event determines a cell's phenotype.
Published Version: doi:10.1126/science.1161427
Other Sources: http://bernstein.harvard.edu/papers/Choi2008.pdf
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:3450063

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  • FAS Scholarly Articles [7594]
    Peer reviewed scholarly articles from the Faculty of Arts and Sciences of Harvard University
 
 

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