A Single-molecule Assay for Telomerase Structure-function Analysis

DSpace/Manakin Repository

A Single-molecule Assay for Telomerase Structure-function Analysis

Citable link to this page

. . . . . .

Title: A Single-molecule Assay for Telomerase Structure-function Analysis
Author: Stone, Michael D.; Wu, John Yanyun; Zhuang, Xiaowei

Note: Order does not necessarily reflect citation order of authors.

Citation: Wu, John Y., Michael D. Stone and Xiaowei Zhuang. 2010. A single-molecule assay for telomerase structure-function analysis. Nucleic Acids Research 38(3): e16.
Full Text & Related Files:
Abstract: The activity of the telomerase ribonucleoprotein enzyme is essential for the maintenance of genome stability and normal cell development. Despite the biomedical importance of telomerase activity, detailed structural models for the enzyme remain to be established. Here we report a single-molecule assay for direct structural analysis of catalytically active telomerase enzymes. In this assay, oligonucleotide hybridization was used to probe the primer-extension activity of individual telomerase enzymes with single nucleotide sensitivity, allowing precise discrimination between inactive, active and processive enzyme binding events. FRET signals from enzyme molecules during the active and processive binding events were then used to determine the global organization of telomerase RNA within catalytically active holoenzymes. Using this assay, we have identified an active conformation of telomerase among a heterogeneous population of enzymes with distinct structures.
Published Version: doi:10.1093/nar/gkp1033
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2817460/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Open Access Policy Articles, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#OAP
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:4454181

Show full Dublin Core record

This item appears in the following Collection(s)

  • FAS Scholarly Articles [7501]
    Peer reviewed scholarly articles from the Faculty of Arts and Sciences of Harvard University
 
 

Search DASH


Advanced Search
 
 

Submitters