Calcium Dynamics During Fertilization in C. elegans

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Calcium Dynamics During Fertilization in C. elegans

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dc.contributor.author Hengartner, Michael O
dc.contributor.author Aravinthan, Samuel
dc.contributor.author Murthy, Venkatesh N.
dc.date.accessioned 2010-09-30T14:31:49Z
dc.date.issued 2001
dc.identifier.citation Samuel, Aravinthan D. T., Venkatesh N. Murthy, and Michael O Hengartner. 2001. Calcium dynamics during fertilization in C. elegans. BMC Developmental Biology 1:8. en_US
dc.identifier.issn 1471-213X en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:4454182
dc.description.abstract Background: Of the animals typically used to study fertilization-induced calcium dynamics, none is as accessible to genetics and molecular biology as the model organism Caenorhabditis elegans. Motivated by the experimental possibilities inherent in using such a well-established model organism, we have characterized fertilization-induced calcium dynamics in C. elegans. Results: Owing to the transparency of the nematode, we have been able to study the calcium signal in C. elegans fertilization in vivo by monitoring the fluorescence of calcium indicator dyes that we introduce into the cytosol of oocytes. In C. elegans, fertilization induces a single calcium transient that is initiated soon after oocyte entry into the spermatheca, the compartment that contains sperm. Therefore, it is likely that the calcium transient is initiated by contact with sperm. This calcium elevation spreads throughout the oocyte, and decays monotonically after which the cytosolic calcium concentration returns to that preceding fertilization. Only this single calcium transient is observed. Conclusion: Development of a technique to study fertilization induced calcium transients opens several experimental possibilities, e.g., identification of the signaling events intervening sperm binding and calcium elevation, identifying the possible roles of the calcium elevation such as the completion of meiosis, the formation of the eggshell, and the establishing of the embryo's axis of symmetry. en_US
dc.description.sponsorship Molecular and Cellular Biology en_US
dc.language.iso en_US en_US
dc.publisher BioMed Central en_US
dc.relation.isversionof doi:10.1186/1471-213X-1-8 en_US
dc.relation.hasversion http://www.ncbi.nlm.nih.gov/pmc/articles/PMC31342/pdf/ en_US
dash.license LAA
dc.title Calcium Dynamics During Fertilization in C. elegans en_US
dc.type Journal Article en_US
dc.description.version Version of Record en_US
dc.relation.journal BMC Developmental Biology en_US
dash.depositing.author Murthy, Venkatesh N.
dc.date.available 2010-09-30T14:31:49Z

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  • FAS Scholarly Articles [7220]
    Peer reviewed scholarly articles from the Faculty of Arts and Sciences of Harvard University

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