A Src-Like Inactive Conformation in the Abl Tyrosine Kinase Domain

DSpace/Manakin Repository

A Src-Like Inactive Conformation in the Abl Tyrosine Kinase Domain

Citable link to this page

. . . . . .

Title: A Src-Like Inactive Conformation in the Abl Tyrosine Kinase Domain
Author: Levinson, Nicholas M; Kuchment, Olga; Shen, Kui; Koldobskiy, Michael; Cole, Philip A; Kuriyan, John; Young, Matthew A.; Karplus, Martin

Note: Order does not necessarily reflect citation order of authors.

Citation: Levinson, Nicholas M., Olga Kuchment, Kui Shen, Matthew A. Young, Michael Koldobskiy, Martin Karplus, Philip A. Cole, and John Kuriyan. 2006. A Src-like inactive conformation in the Abl tyrosine kinase domain. PLoS Biology 4(5): e144.
Full Text & Related Files:
Abstract: The improper activation of the Abl tyrosine kinase results in chronic myeloid leukemia (CML). The recognition of an inactive conformation of Abl, in which a catalytically important Asp-Phe-Gly (DFG) motif is flipped by approximately 180° with respect to the active conformation, underlies the specificity of the cancer drug imatinib, which is used to treat CML. The DFG motif is not flipped in crystal structures of inactive forms of the closely related Src kinases, and imatinib does not inhibit c-Src. We present a structure of the kinase domain of Abl, determined in complex with an ATP–peptide conjugate, in which the protein adopts an inactive conformation that resembles closely that of the Src kinases. An interesting aspect of the Src-like inactive structure, suggested by molecular dynamics simulations and additional crystal structures, is the presence of features that might facilitate the flip of the DFG motif by providing room for the phenylalanine to move and by coordinating the aspartate side chain as it leaves the active site. One class of mutations in BCR–Abl that confers resistance to imatinib appears more likely to destabilize the inactive Src-like conformation than the active or imatinib-bound conformations. Our results suggest that interconversion between distinctly different inactive conformations is a characteristic feature of the Abl kinase domain.
Published Version: doi:10.1371/journal.pbio.0040144
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1450098/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:4454683

Show full Dublin Core record

This item appears in the following Collection(s)

  • FAS Scholarly Articles [6463]
    Peer reviewed scholarly articles from the Faculty of Arts and Sciences of Harvard University

Search DASH

Advanced Search