Browsing Harvard Medical School by Title

Now showing items 2493-2512 of 18428

    • Characterization of a Nucleotide Kinase Encoded by Bacteriophage T7 

      Tran, Ngoc Q.; Tabor, Stanley; Amarasiriwardena, Chitra J.; Kulczyk, Arkadiusz W.; Richardson, Charles C. (American Society for Biochemistry and Molecular Biology, 2012)
      Gene 1.7 protein is the only known nucleotide kinase encoded by bacteriophage T7. The enzyme phosphorylates dTMP and dGMP to dTDP and dGDP, respectively, in the presence of a phosphate donor. The phosphate donors are dTTP, ...

    • Characterization of a Replication-Competent, Integrase-Defective Human Immunodeficiency Virus (HIV)/Simian Virus 40 Chimera as a Powerful Tool for the Discovery and Validation of HIV Integrase Inhibitors 

      Daelemans, Dirk; Lu, Richard; De Clercq, Erik; Engelman, Alan (American Society for Microbiology, 2007)
      Integrase is actively studied as an antiviral target, but many inhibitors selected from biochemical screens fail to inhibit human immunodeficiency virus (HIV) replication or primarily affect off-site targets. Here we develop ...

    • Characterization of a Spontaneous Retinal Neovascular Mouse Model 

      Hasegawa, Eiichi; Sweigard, Harry; Husain, Deeba; Olivares, Ana M.; Chang, Bo; Smith, Kaylee E.; Birsner, Amy E.; D’Amato, Robert J.; Michaud, Norman A.; Han, Yinan; Vavvas, Demetrios G.; Miller, Joan W.; Haider, Neena B.; Connor, Kip M. (Public Library of Science, 2014)
      Background: Vision loss due to vascular disease of the retina is a leading cause of blindness in the world. Retinal angiomatous proliferation (RAP) is a subgroup of neovascular age-related macular degeneration (AMD), whereby ...

    • Characterization of activating mutations of NOTCH3 in T cell acute lymphoblastic leukemia and anti-leukemic activity of NOTCH3 inhibitory antibodies 

      Bernasconi-Elias, Paula; Hu, Tiancen; Jenkins, David; Firestone, Brant; Gans, Sara; Kurth, Esther; Capodieci, Paola; Deplazes-Lauber, Joelle; Petropoulos, Konstantin; Thiel, Phillip; Ponsel, Dirk; Choi, Sung Hee; LeMotte, Peter; London, Anne; Goetcshkes, Margaret; Nolin, Erin; Jones, Michael D.; Slocum, Kelly; Kluk, Michael J.; Weinstock, David M.; Christodoulou, Alexandra; Weinberg, Olga; Jaehrling, Jan; Ettenberg, Seth A.; Buckler, Alan; Blacklow, Stephen C.; Aster, Jon C.; Fryer, Christy J. (2016)
      Notch receptors have been implicated as oncogenic drivers in several cancers, the most notable example being NOTCH1 in T-cell acute lymphoblastic leukemia (T-ALL). To characterize the role of activated NOTCH3 in cancer, ...

    • Characterization of Adherent Bacteroidales from Intestinal Biopsies of Children and Young Adults with Inflammatory Bowel Disease 

      Zitomersky, Naamah L.; Atkinson, Benjamin J.; Franklin, Sarah W.; Mitchell, Paul D.; Snapper, Scott B.; Comstock, Laurie E.; Bousvaros, Athos (Public Library of Science, 2013)
      There is extensive evidence implicating the intestinal microbiota in inflammatory bowel disease [IBD], but no microbial agent has been identified as a sole causative agent. Bacteroidales are numerically dominant intestinal ...

    • Characterization of aeroallergen sensitivities in children with allergic rhinitis and chronic rhinosinusitis 

      Sedaghat, Ahmad R.; Phipatanakul, Wanda; Cunningham, Michael J. (OceanSide Publications, Inc., 2014)
      Allergic rhinitis is a common comorbid condition in pediatric chronic rhinosinusitis (CRS). Testing for aeroallergen sensitization should therefore be considered in the evaluation of children with CRS. At present the ...

    • Characterization of Age-Dependent and Progressive Cortical Neuronal Degeneration in Presenilin Conditional Mutant Mice 

      Wines-Samuelson, Mary; Schulte, Eva C.; Smith, Miriam J.; Aoki, Chiye; Liu, Xinran; Cookson, Mark R.; Kelleher, Raymond J.; Shen, Jie (Public Library of Science, 2010)
      Presenilins are the major causative genes of familial Alzheimer's disease (AD). Our previous study has demonstrated essential roles of presenilins in memory and neuronal survival. Here, we explore further how loss of ...

    • Characterization of an Acute Muscle Contraction Model Using Cultured C2C12 Myotubes 

      Manabe, Yasuko; Miyatake, Shouta; Takagi, Mayumi; Nakamura, Mio; Okeda, Ai; Nakano, Taemi; Hirshman, Michael F.; Goodyear, Laurie Joy; Fujii, Nobuharu L. (Public Library of Science, 2012)
      A cultured C2C12 myotube contraction system was examined for application as a model for acute contraction-induced phenotypes of skeletal muscle. C2C12 myotubes seeded into 4-well rectangular plates were placed in a contraction ...

    • Characterization of Anti-Salmonella enterica Serotype Typhi Antibody Responses in Bacteremic Bangladeshi Patients by an Immunoaffinity Proteomics-Based Technology 

      Charles, Richelle C.; Sheikh, Alaullah; Krastins, Bryan; Harris, Jason B.; Bhuiyan, M. Saruar; LaRocque, Regina C.; Logvinenko, Tanya; Sarracino, David A.; Kudva, Indira T.; Eisenstein, Jana; Podolsky, Michael J.; Kalsy, Anuj; Brooks, W. Abdullah; Ludwig, Albrecht; John, Manohar; Calderwood, Stephen B.; Qadri, Firdausi; Ryan, Edward T. (American Society for Microbiology, 2010)
      Salmonella enterica serotype Typhi is the cause of typhoid fever and a human-restricted pathogen. Currently available typhoid vaccines provide 50 to 90% protection for 2 to 5 years, and available practical diagnostic assays ...

    • Characterization of Bipolar Disorder Patient-Specific Induced Pluripotent Stem Cells from a Family Reveals Neurodevelopmental and mRNA Expression Abnormalities 

      Madison, Jon M.; Zhou, Fen; Nigam, Aparna; Hussain, Ali; Barker, Douglas D.; Nehme, Ralda; van der Ven, Karlijn; Hsu, Jenny; Wolf, Pavlina; Fleishman, Morgan; O’Dushlaine, Colm; Rose, Sam; Chambert, Kimberly; Lau, Frank H.; Ahfeldt, Tim; Rueckert, Erroll H.; Sheridan, Steven D.; Fass, Daniel M.; Nemesh, James; Mullen, Thomas E.; Daheron, Laurence; McCarroll, Steve; Sklar, Pamela; Perlis, Roy H.; Haggarty, Stephen J. (2014)
      Bipolar disorder (BD) is a common neuropsychiatric disorder characterized by chronic recurrent episodes of depression and mania. Despite evidence for high heritability of BD, little is known about its underlying pathophysiology. ...

    • Characterization of CD8+ T Cell Differentiation following SIVΔnef Vaccination by Transcription Factor Expression Profiling 

      Billingsley, James M.; Rajakumar, Premeela A.; Connole, Michelle A.; Salisch, Nadine C.; Adnan, Sama; Kuzmichev, Yury V.; Hong, Henoch S.; Reeves, R. Keith; Kang, Hyung-joo; Li, Wenjun; Li, Qingsheng; Haase, Ashley T.; Johnson, R. Paul (Public Library of Science, 2015)
      The onset of protective immunity against pathogenic SIV challenge in SIVΔnef-vaccinated macaques is delayed for 15-20 weeks, a process that is related to qualitative changes in CD8+ T cell responses induced by SIVΔnef. As ...

    • Characterization of cells from patient-derived fibrovascular membranes in proliferative diabetic retinopathy 

      Kim, Leo A.; Wong, Lindsay L.; Amarnani, Dhanesh S.; Bigger-Allen, Alexander A.; Hu, Yang; Marko, Christina K.; Eliott, Dean; Shah, Vinay A.; McGuone, Declan; Stemmer-Rachamimov, Anat O.; Gai, Xiaowu; D’Amore, Patricia A.; Arboleda-Velasquez, Joseph F. (Molecular Vision, 2015)
      Purpose Epiretinal fibrovascular membranes (FVMs) are a hallmark of proliferative diabetic retinopathy (PDR). Surgical removal of FVMs is often indicated to treat tractional retinal detachment. This potentially informative ...

    • Characterization of Circulating Natural Killer Cells in Neotropical Primates 

      Carville, Angela; Evans, Tristan I.; Reeves, R. Keith (Public Library of Science, 2013)
      Despite extensive use of nonhuman primates as models for infectious diseases and reproductive biology, imprecise phenotypic and functional definitions exist for natural killer (NK) cells. This deficit is particularly ...

    • Characterization of Conserved Toxicogenomic Responses in Chemically Exposed Hepatocytes across Species and Platforms 

      El-Hachem, Nehme; Grossmann, Patrick; Blanchet-Cohen, Alexis; Bateman, Alain R.; Bouchard, Nicolas; Archambault, Jacques; Aerts, Hugo J.W.L.; Haibe-Kains, Benjamin (National Institute of Environmental Health Sciences, 2015)
      Background: Genome-wide expression profiling is increasingly being used to identify transcriptional changes induced by drugs and environmental stressors. In this context, the Toxicogenomics Project–Genomics Assisted Toxicity ...

    • Characterization of Cre recombinase models for the study of adipose tissue 

      Jeffery, Elise; Berry, Ryan; Church, Christopher D; Yu, Songtao; Shook, Brett A; Horsley, Valerie; Rosen, Evan D; Rodeheffer, Matthew S (Landes Bioscience, 2014)
      The study of adipose tissue in vivo has been significantly advanced through the use of genetic mouse models. While the aP2-CreBI and aP2-CreSalk lines have been widely used to target adipose tissue, the specificity of these ...

    • Characterization of CSF2RA mutation related juvenile pulmonary alveolar proteinosis 

      Hildebrandt, Jenna; Yalcin, Ebru; Bresser, Hans-Georg; Cinel, Guzin; Gappa, Monika; Haghighi, Alireza; Kiper, Nural; Khalilzadeh, Soheila; Reiter, Karl; Sayer, John; Schwerk, Nicolaus; Sibbersen, Anke; Van Daele, Sabine; Nübling, Georg; Lohse, Peter; Griese, Matthias (BioMed Central, 2014)
      Background: Juvenile pulmonary alveolar proteinosis (PAP) due to CSF2RA mutations is a rare disorder with only a few cases described worldwide. Methods: We identified nine children with severe diffuse interstitial lung ...

    • Characterization of DDR2 Inhibitors for the Treatment of DDR2 Mutated Nonsmall Cell Lung Cancer 

      Terai, Hideki; Tan, Li; Beauchamp, Ellen M.; Hatcher, John M.; Liu, Qingsong; Meyerson, Matthew; Gray, Nathanael S.; Hammerman, Peter S. (American Chemical Society, 2015)
      Despite advances in precision medicine approaches over the past decade, the majority of nonsmall cell lung cancers (NSCLCs) are refractory to treatment with targeted small molecule inhibitors. Previous work has identified ...

    • Characterization of Defects in Ion Transport and Tissue Development in Cystic Fibrosis Transmembrane Conductance Regulator (CFTR)-Knockout Rats 

      Tuggle, Katherine L.; Birket, Susan E.; Cui, Xiaoxia; Hong, Jeong; Warren, Joe; Reid, Lara; Chambers, Andre; Ji, Diana; Gamber, Kevin; Chu, Kengyeh K.; Tearney, Guillermo; Tang, Li Ping; Fortenberry, James A.; Du, Ming; Cadillac, Joan M.; Bedwell, David M.; Rowe, Steven M.; Sorscher, Eric J.; Fanucchi, Michelle V. (Public Library of Science, 2014)
      Animal models for cystic fibrosis (CF) have contributed significantly to our understanding of disease pathogenesis. Here we describe development and characterization of the first cystic fibrosis rat, in which the cystic ...

    • Characterization of Early Steps in the Poliovirus Infection Process: Receptor-Decorated Liposomes Induce Conversion of the Virus to Membrane-Anchored Entry-Intermediate Particles 

      Tuthill, Tobias J.; Bubeck, Doryen; Rowlands, David J.; Hogle, James M. (American Society for Microbiology, 2006)
      The mechanism by which poliovirus infects the cell has been characterized by a combination of biochemical and structural studies, leading to a working model for cell entry. Upon receptor binding at physiological temperature, ...

    • Characterization of Effector T Cells in Dry Eye Disease 

      Annan, Jaafar; Chauhan, Sunil; Ecoiffier, Tatiana; Zhang, Qiang; Saban, Daniel; Dana, Reza (Association for Research in Vision and Ophthalmology (ARVO), 2009)
      purpose. Dry eye disease (DED) is associated with ocular surface inflammation that is thought to be mediated primarily by CD4 T cells. The purpose of this study was to investigate whether this T cell–mediated immune response ...