Now showing items 3713-3732 of 18424

    • Creating Critical Consumers of Health and Science News: Teaching Science to the Non-Scientist Using Newsworthy Topics in the Life Sciences† 

      Coderre, Raymond W.; Uekermann, Kristen A.; Choi, Youngeun; Anderson, William J. (American Society of Microbiology, 2016)
      Scientists constantly make groundbreaking discoveries, some of which receive attention from the press. We designed a course intended for a lay audience that provides the scientific background to appreciate these reports ...
    • Creation and implementation of SAMPRO™: A school-based asthma management program 

      Lemanske, Robert F.; Kakumanu, Sujani; Shanovich, Kathleen; Antos, Nicholas; Cloutier, Michelle M.; Mazyck, Donna; Phipatanakul, Wanda; Schantz, Shirley; Szefler, Stanley; Vandlik, Renee; Williams, Paul (2016)
      Clinicians who care for children with asthma have an obligation to coordinate asthma care with the schools. Aside from routine clinical care of asthmatic children, providers must educate the family and child about the need ...
    • Creation of an Open-Access, Mutation-Defined Fibroblast Resource for Neurological Disease Research 

      Wray, Selina; Self, Matthew; Lewis, Patrick A.; Taanman, Jan-Willem; Ryan, Natalie S.; Mahoney, Colin J.; Liang, Yuying; Devine, Michael J.; Sheerin, Una-Marie; Houlden, Henry; Morris, Huw R.; Healy, Daniel; Marti-Masso, Jose-Felix; Preza, Elisavet; Barker, Suzanne; Sutherland, Margaret; Corriveau, Roderick A.; D'Andrea, Michael; Schapira, Anthony H. V.; Uitti, Ryan J.; Guttman, Mark; Opala, Grzegorz; Jasinska-Myga, Barbara; Puschmann, Andreas; Nilsson, Christer; Espay, Alberto J.; Slawek, Jaroslaw; Gutmann, Ludwig; Boeve, Bradley F.; Boylan, Kevin; Stoessl, A. Jon; Ross, Owen A.; Maragakis, Nicholas J.; Van Gerpen, Jay; Gerstenhaber, Melissa; Gwinn, Katrina; Dawson, Ted M.; Marder, Karen S.; Clark, Lorraine N.; Przedborski, Serge E.; Finkbeiner, Steven; Rothstein, Jeffrey D.; Wszolek, Zbigniew K.; Rossor, Martin N.; Hardy, John; Isacson, Ole Stefan (Public Library of Science, 2012)
      Our understanding of the molecular mechanisms of many neurological disorders has been greatly enhanced by the discovery of mutations in genes linked to familial forms of these diseases. These have facilitated the generation ...
    • The crisis of patient-physician trust and bioethics: lessons and inspirations from China 

      Nie, Jing-Bao; Li, Lun; Gillett, Grant; Tucker, Joseph D.; Kleinman, Arthur Michael (Wiley-Blackwell, 2017)
      Trust is indispensable not only for interpersonal relationships and social life, but for good quality healthcare. As manifested in the increasing violence and tension in patient-physician relationships, China has been ...
    • CRISPR Display: A modular method for locus-specific targeting of long noncoding RNAs and synthetic RNA devices in vivo 

      Shechner, David M.; Hacisüleyman, Ezgi; Younger, Scott T.; Rinn, John L. (2016)
      Noncoding RNAs (ncRNAs) comprise an important class of regulatory molecules that mediate a vast array of biological processes. This broad functional capacity has also facilitated the design of artificial ncRNAs with novel ...
    • CRISPR DNA Base Editors With Reduced RNA Off-Target and Self-Editing Activities 

      Grünewald, Julian; Zhou, Ronghao; Iyer, Sowmya; Lareau, Caleb; Garcia, Sara; Aryee, Martin; Joung, Keith (Springer Science and Business Media LLC, 2019-09)
      Cytosine or adenine base editors (CBEs or ABEs) can introduce specific DNA C-to-T or A-to-G alterations1,2,3,4. However, we recently demonstrated that they can also induce transcriptome-wide guide-RNA-independent editing ...
    • CRISPR Guide RNA Design for Research Applications 

      Mohr, Stephanie; Hu, Yanhui; Ewen-Campen, Benjamin; Housden, Benjamin; Viswanatha, Raghuvir; Perrimon, Norbert (Wiley-Blackwell, 2016-09)
      The rapid rise of CRISPR as a technology for genome engineering and related research applications has created a need for algorithms and associated online tools that facilitate design of on‐target and effective guide RNAs ...
    • CRISPR RNA-guided activation of endogenous human genes 

      Maeder, Morgan L; Linder, Samantha J; Cascio, Vincent M; Fu, Yanfang; Ho, Quan H; Joung, J Keith (2013)
      Catalytically inactive CRISPR-associated 9 nuclease (dCas9) can be directed by short guide RNAs (gRNAs) to repress endogenous genes in bacteria and human cells. Here we show that a dCas9-VP64 transcriptional activation ...
    • CRISPR-Cas-amplified urinary biomarkers for multiplexed and portable cancer diagnostics 

      Hao, Liangliang; Zhao, Renee T.; Welch, Nicole; Tan, Edward Kah Wei; Zhong, Qian; Harzallah, Nour Saida; Ngambenjawong, Chayanon; Ko, Henry; Fleming, Heather E.; Sabeti, Pardis C.; Bhatia, Sangeeta N. (Springer Science and Business Media LLC, 2023-04-24)
      Synthetic biomarkers, exogenous probes that generate molecular reporters, represent an emerging paradigm in precision diagnostics with applications across infectious and noncommunicable diseases. These methods use multiplexing ...
    • CRISPR-Cas: To Take Up DNA or Not—That Is the Question 

      Weinberger, Ariel D; Gilmore, Michael S. (Elsevier BV, 2012)
      Historically landmark experiments showed that capsule switching is critical for Streptococcus pneumonia survival. Further studies demonstrated that capsule ‘transformation’ occurs via DNA uptake. In this issue of Cell Host ...
    • CRISPR-mediated direct mutation of cancer genes in the mouse liver 

      Xue, Wen; Chen, Sidi; Yin, Hao; Tammela, Tuomas; Papagiannakopoulos, Thales; Joshi, Nikhil S.; Cai, Wenxin; Yang, Gillian; Bronson, Roderick; Crowley, Denise G.; Zhang, Feng; Anderson, Daniel G.; Sharp, Phillip A.; Jacks, Tyler (2014)
      The study of cancer genes in mouse models has traditionally relied on genetically-engineered strains made via transgenesis or gene targeting in embryonic stem (ES) cells1. Here we describe a new method of cancer model ...
    • CRISPR/Cas9-Mediated Gene Knock-Down in Post-Mitotic Neurons 

      Straub, Christoph; Granger, Adam J.; Saulnier, Jessica L.; Sabatini, Bernardo L. (Public Library of Science, 2014)
      The prokaryotic adaptive immune system CRISPR/Cas9 has recently been adapted for genome editing in eukaryotic cells. This technique allows for sequence-specific induction of double-strand breaks in genomic DNA of individual ...
    • CRISPR/Cas9-Mediated Phage Resistance Is Not Impeded by the DNA Modifications of Phage T4 

      Yaung, Stephanie J.; Esvelt, Kevin M.; Church, George M. (Public Library of Science, 2014)
      Bacteria rely on two known DNA-level defenses against their bacteriophage predators: restriction-modification and Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR-associated (Cas) systems. Certain ...
    • Cristae remodeling causes acidification detected by integrated graphene sensor during mitochondrial outer membrane permeabilization 

      Pham, Ted D.; Pham, Phi Q.; Li, Jinfeng; Letai, Anthony G.; Wallace, Douglas C.; Burke, Peter J. (Nature Publishing Group, 2016)
      The intrinsic apoptotic pathway and the resultant mitochondrial outer membrane permeabilization (MOMP) via BAK and BAX oligomerization, cytochrome c (cytc) release, and caspase activation are well studied, but their effect ...
    • Criteria for assessing high-priority drug-drug interactions for clinical decision support in electronic health records 

      Phansalkar, Shobha; Desai, Amrita; Choksi, Anish; Yoshida, Eileen; Doole, John; Czochanski, Melissa; Tucker, Alisha D; Middleton, Blackford; Bell, Douglas; Bates, David W (BioMed Central, 2013)
      Background: High override rates for drug-drug interaction (DDI) alerts in electronic health records (EHRs) result in the potentially dangerous consequence of providers ignoring clinically significant alerts. Lack of ...
    • Criteria for Clinically Relevant Weakness and Low Lean Mass and Their Longitudinal Association With Incident Mobility Impairment and Mortality: The Foundation for the National Institutes of Health (FNIH) Sarcopenia Project 

      McLean, Robert R.; Shardell, Michelle D.; Alley, Dawn E.; Cawthon, Peggy M.; Fragala, Maren S.; Harris, Tamara B.; Kenny, Anne M.; Peters, Katherine W.; Ferrucci, Luigi; Guralnik, Jack M.; Kritchevsky, Stephen B.; Kiel, Douglas P.; Vassileva, Maria T.; Xue, Qian-Li; Perera, Subashan; Studenski, Stephanie A.; Dam, Thuy-Tien L. (Oxford University Press, 2014)
      Background. This analysis sought to determine the associations of the Foundation for the National Institutes of Health Sarcopenia Project criteria for weakness and low lean mass with likelihood for mobility impairment (gait ...
    • Critical Endothelial Regulation by LRP5 during Retinal Vascular Development 

      Huang, Wei; Li, Qing; Amiry-Moghaddam, Mahmood; Hokama, Madoka; Sardi, Sylvia H.; Nagao, Masashi; Warman, Matthew L.; Olsen, Bjorn R. (Public Library of Science, 2016)
      Vascular abnormalities in the eye are the leading cause of many forms of inherited and acquired human blindness. Loss-of-function mutations in the Wnt-binding co-receptor LRP5 leads to aberrant ocular vascularization and ...
    • CRITICAL ILLNESS METABOLOMICS REGARDING INFLAMMATION AND KIDNEY DYSFUNCTION: POST-HOC ANALYSES OF THE VITDAL-ICU TRIAL 

      Kobayashi, Hirotada (2021-05-14)
      OVERVIEW: During the Master of Medical Sciences program at Harvard Medical School, I have focused on the metabolomics of critical illness. Critical illness is a life-threatening condition and presents high hospital ...
    • A Critical Period for Functional Motor Recovery After Peripheral Nerve Injury in the Mouse 

      Lee, Stella Joonmyung (2014-07-07)
      Repair of peripheral nerve injury often results in poor functional motor recovery. This deficit has previously been attributed to the failure of axons to regenerate into the muscle. However, we have recently reported that ...
    • Critical phosphoprotein elements that regulate polymerase architecture and function in vesicular stomatitis virus 

      Rahmeh, Amal; Morin, Benjamin; Schenk, Andreas; Liang, Bo; Heinrich, Bianca; Brusic, Vesna; Walz, Thomas; Whelan, Sean (National Academy of Sciences, 2012)
      The RNA-dependent RNA polymerase (RdRP) of nonsegmented negative-sense RNA viruses consists of a large catalytic protein (L) and a phosphoprotein cofactor (P). During infection, the RdRP replicates and transcribes the viral ...