Browsing Harvard Medical School by Title
Now showing items 4886-4905 of 18428
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Driving with Central Visual Field Loss II: How Scotomas above or below the Preferred Retinal Locus (PRL) Affect Hazard Detection in a Driving Simulator
(Public Library of Science, 2015)We determined whether binocular central scotomas above or below the preferred retinal locus affect detection of hazards (pedestrians) approaching from the side. Seven participants with central field loss (CFL), and seven ... -
Drop-on-Demand Single Cell Isolation and Total RNA Analysis
(Public Library of Science, 2011)Technologies that rapidly isolate viable single cells from heterogeneous solutions have significantly contributed to the field of medical genomics. Challenges remain both to enable efficient extraction, isolation and ... -
A droplet digital PCR detection method for rare L1 insertions in tumors
(BioMed Central, 2014)Background: The active human mobile element, long interspersed element 1 (L1) currently populates human genomes in excess of 500,000 copies per haploid genome. Through its mobility via a process called target primed reverse ... -
Droplet-Based Combinatorial Indexing for Massive-Scale Single-Cell Chromatin Accessibility
(Springer Science and Business Media LLC, 2019-06-24)Recent technical advancements have facilitated the mapping of epigenomes at single-cell resolution; however, the throughput and quality of these methods have limited their widespread adoption. Here we describe a high-quality ... -
Drosophila and the genetics of the internal milieu
(2007)'Homeostasis', from the Greek words for 'same' and 'steady', refers to ways in which the body acts to maintain a stable internal environment despite perturbations. Recent studies in Drosophila exemplify the conservation ... -
Drosophila as a Model for Context-Dependent Tumorigenesis
(Wiley, 2014-01)Drosophila can exhibit classic hallmarks of cancer, such as evasion of apoptosis, sustained proliferation, metastasis, prolonged survival, genome instability, and metabolic reprogramming, when cancer-related genes are ... -
Drosophila as a Model for Interorgan Communication: Lessons from Studies on Energy Homeostasis
(Elsevier BV, 2011-07-19)Current studies of physiological communication between Drosophila organs are beginning to address the fundamental problem of how nutrients regulate organismal growth, stem cell behavior, immunity, and aging. Advances in ... -
Drosophila as a model system to study autophagy
(Springer Verlag, 2010)Originally identified as a response to starvation in yeast, autophagy is now understood to fulfill a variety of roles in higher eukaryotes, from the maintenance of cellular homeostasis to the cellular response to stress, ... -
Drosophila Cytokine Unpaired 2 Regulates Physiological Homeostasis by Remotely Controlling Insulin Secretion
(Elsevier BV, 2012-09-28)In Drosophila the fat body (FB), a functional analog of the vertebrate adipose tissue, is the 'nutrient sensor' that conveys the nutrient status to the insulin producing cells (IPCs) in the fly brain to release insulin-like ... -
The Drosophila Forkhead Transcription Factor FOXO Mediates the Reduction in Cell Number Associated with Reduced Insulin Signaling
(BioMed Central, 2003)Background: Forkhead transcription factors belonging to the FOXO subfamily are negatively regulated by protein kinase B (PKB) in response to signaling by insulin and insulin-like growth factor in Caenorhabditis elegans and ... -
A Drosophila gene expressed in the embryonic CNS shares one conserved domain with the mammalian GAP-43
(The Company of Biologists, 1989-03-01)By cross hybridization with the mammalian growth-related protein, GAP-43, we have isolated several Drosophila cDNAs and genomic sequences. These sequences correspond to a single copy gene that encodes two developmentally ... -
Drosophila glucome screening identifies Ck1alpha as a regulator of mammalian glucose metabolism
(Nature Pub. Group, 2015)Circulating carbohydrates are an essential energy source, perturbations in which are pathognomonic of various diseases, diabetes being the most prevalent. Yet many of the genes underlying diabetes and its characteristic ... -
Drosophila Growth Cones Advance by Forward Translocation of the Neuronal Cytoskeletal Meshwork In Vivo
(Public Library of Science, 2013)In vitro studies conducted in Aplysia and chick sensory neurons indicate that in addition to microtubule assembly, long microtubules in the C-domain of the growth cone move forward as a coherent bundle during axonal ... -
Drosophila Heparan Sulfate, a Novel Design
(Elsevier BV, 2012-06-22)Heparan sulfate (HS) proteoglycans play critical roles in a wide variety of biological processes such as growth factor signaling, cell adhesion, wound healing, and tumor metastasis. Functionally important interactions ... -
Drosophila Host Model Reveals New Enterococcus faecalis Quorum-Sensing Associated Virulence Factors
(Public Library of Science, 2013)Enterococcus faecalis V583 is a vancomycin-resistant clinical isolate which belongs to the hospital-adapted clade, CC2. This strain harbours several factors that have been associated with virulence, including the fsr ... -
Drosophila Intestinal Stem and Progenitor Cells Are Major Sources and Regulators of Homeostatic Niche Signals
(National Academy of Sciences, 2018-11-27)Epithelial homeostasis requires the precise balance of epithelial stem/progenitor proliferation and differentiation. While many signaling pathways that regulate epithelial stem cells have been identified, it is probable ... -
Drosophila Jun Relays the Jun Amino-Terminal Kinase Signal Transduction Pathway to the Decapentaplegic Signal Transduction Pathway in Regulating Epithelial Cell Sheet Movement
(Cold Spring Harbor Laboratory, 1997-07-01)We have characterized mutations in the Drosophila homolog of the mammalian proto-oncogene c-Jun gene (Djun). We demonstrate that DJUN in the embryo is a downstream target of the JNK signal transduction pathway during dorsal ... -
Drosophila Protein interaction Map (DPiM): A paradigm for metazoan protein complex interactions
(Landes Bioscience, 2012)Proteins perform essential cellular functions as part of protein complexes, often in conjunction with RNA, DNA, metabolites and other small molecules. The genome encodes thousands of proteins but not all of them are expressed ... -
A Drosophila screen identifies neurofibromatosis-1 genetic modifiers involved in systemic and synaptic growth
(Landes Bioscience, 2014)Neurofibromatosis type 1 (NF1) is caused by loss of a negative regulator of Ras oncoproteins. Unknown genetic modifiers have been implicated in NF1’s characteristic variability. Drosophila melanogaster dNf1 phenotypes ... -
Drosophila selenophosphate synthetase 1 regulates vitamin B6 metabolism: prediction and confirmation
(BMC, 2011)Background: There are two selenophosphate synthetases (SPSs) in higher eukaryotes, SPS1 and SPS2. Of these two isotypes, only SPS2 catalyzes selenophosphate synthesis. Although SPS1 does not contain selenophosphate synthesis ...