Browsing Harvard Medical School by Title
Now showing items 6501-6520 of 18424
-
Fixing DNA breaks during class switch recombination
(Rockefeller University Press, 2008)Immunoglobulin (Ig) class switch recombination (CSR) involves the breakage and subsequent repair of two DNA sequences, known as switch (S) regions, which flank IgH constant region exons. The resolution of CSR-associated ... -
FLAG Assay as a Novel Method for Real-Time Signal Generation During PCR: Application to Detection and Genotyping of KRAS Codon 12 Mutations
(Oxford University Press, 2007)Real-time signal generation methods for detection and characterization of low-abundance mutations in genomic DNA are powerful tools for cancer diagnosis and prognosis. Mutations in codon 12 of the oncogene KRAS, for example, ... -
Flagellar Motility Is Critical for Listeria monocytogenes Biofilm Formation
(American Society for Microbiology, 2007)The food-borne pathogen Listeria monocytogenes attaches to environmental surfaces and forms biofilms that can be a source of food contamination, yet little is known about the molecular mechanisms of its biofilm development. ... -
Flagellin-Deficient Legionella Mutants Evade Caspase-1- and Naip5-Mediated Macrophage Immunity
(Public Library of Science, 2006)Macrophages from C57BL/6J (B6) mice restrict growth of the intracellular bacterial pathogen Legionella pneumophila. Restriction of bacterial growth requires caspase-1 and the leucine-rich repeat-containing protein Naip5 ... -
Flap Endonuclease Activity of Gene 6 Exonuclease of Bacteriophage T7
(American Society for Biochemistry and Molecular Biology, 2014)Background: Flap endonucleases remove 5-single-stranded DNA termini. Results: T7 gene 6 exonuclease is a flap endonuclease that cleaves 5-single-stranded termini one nucleotide into the duplex. Conclusion: The flap ... -
Flap endonuclease of bacteriophage T7: Possible roles in RNA primer removal, recombination and host DNA breakdown
(Landes Bioscience, 2014)Gene 6 protein of bacteriophage T7 has 5′-3′-exonuclease activity specific for duplex DNA. We have found that gene 6 protein also has flap endonuclease activity. The flap endonuclease activity is considerably weaker than ... -
Flare frequency, healthcare resource utilisation and costs among patients with gout in a managed care setting: a retrospective medical claims-based analysis
(BMJ Publishing Group, 2015)Objectives: For most gout patients, excruciatingly painful gout attacks are the major clinical burden of the disease. The goal of this study was to assess the association of frequent gout flares with healthcare burden, and ... -
Flash Memory: Photochemical Imprinting of Neuronal Action Potentials onto a Microbial Rhodopsin
(American Chemical Society, 2014)We developed a technique, “flash memory”, to record a photochemical imprint of the activity state—firing or not firing—of a neuron at a user-selected moment in time. The key element is an engineered microbial rhodopsin ... -
Flavanols, Mild Cognitive Impairment, and Alzheimer’s Dementia
(2008-04-15)Alzheimer’s disease (AD) is a dementing neurological disorder that results in progressive memory loss and cognitive decline thought to be associated with buildup of amyloid plaques and neurofibrillary tangles in the brain. ... -
Flavin-containing monooxygenase 3 as a potential player in diabetes-associated atherosclerosis
(Nature Pub. Group, 2015)Despite the well-documented association between insulin resistance and cardiovascular disease, the key targets of insulin relevant to the development of cardiovascular disease are not known. Here, using non-biased profiling ... -
Flavonoid Ampelopsin Inhibits the Growth and Metastasis of Prostate Cancer In Vitro and in Mice
(Public Library of Science, 2012)The objective of this study was to evaluate the chemopreventive effect of a novel flavonoid, ampelopsin (AMP) on the growth and metastasis of prostate cancer cells. AMP showed the more potent activity in inhibiting the ... -
Flavonoid Apigenin Is an Inhibitor of the NAD+ase CD38: Implications for Cellular NAD+ Metabolism, Protein Acetylation, and Treatment of Metabolic Syndrome
(American Diabetes Association, 2013)Metabolic syndrome is a growing health problem worldwide. It is therefore imperative to develop new strategies to treat this pathology. In the past years, the manipulation of NAD+ metabolism has emerged as a plausible ... -
Flavonoid intake and ovarian cancer risk in a population-based case-control study
(Wiley-Blackwell, 2009)Several recent studies have evaluated the association between dietary flavonoid intake and ovarian cancer risk, and all reported significant or suggestive inverse associations with certain flavonoids or flavonoid subclasses; ... -
Flavoring Chemicals in E-Cigarettes: Diacetyl, 2,3-Pentanedione, and Acetoin in a Sample of 51 Products, Including Fruit-, Candy-, and Cocktail-Flavored E-Cigarettes
(National Institute of Environmental Health Sciences, 2015)Background: There are > 7,000 e-cigarette flavors currently marketed. Flavoring chemicals gained notoriety in the early 2000s when inhalation exposure of the flavoring chemical diacetyl was found to be associated with a ... -
Flexible Promoter Architecture Requirements for Coactivator Recruitment
(BioMed Central, 2006)Background: The spatial organization of transcription factor binding sites in regulatory DNA, and the composition of intersite sequences, influences the assembly of the multiprotein complexes that regulate RNA polymerase ... -
Flexible transbronchial optical frequency domain imaging smart needle for biopsy guidance
(Optical Society of America, 2012)Transbronchial needle aspiration (TBNA) is a procedure routinely performed to diagnose peripheral pulmonary lesions. However, TBNA is associated with a low diagnostic yield due to inappropriate needle placement. We have ... -
A Flexibly Shaped Space-time Scan Statistic for Disease Outbreak Detection and Monitoring
(BioMed Central, 2008)Background: Early detection of disease outbreaks enables public health officials to implement disease control and prevention measures at the earliest possible time. A time periodic geographical disease surveillance system ... -
FLEXIQinase, a mass spectrometry-based assay, to unveil multi-kinase mechanisms
(2013)We introduce a mass spectrometry-based method that provides residue-resolved quantitative information about protein phosphorylation. In this FLEXIQinase assay we combined our Full-Length Expressed Stable Isotope-labeled ... -
Flightless-I regulates proinflammatory caspases by selectively modulating intracellular localization and caspase activity
(Rockefeller University Press, 2008)Caspase-1 and caspase-11 are proinflammatory caspases that regulate cytokine production and leukocyte migration during pathogen infection. In an attempt to identify new intracellular regulators of caspase-11, we found that ... -
Flip-Flop HSV-BAC: Bacterial Artificial Chromosome Based System for Rapid Generation of Recombinant Herpes Simplex Virus Vectors Using Two Independent Site-specific Recombinases
(BioMed Central, 2006)Background: Oncolytic herpes simplex virus (HSV) vectors that specifically replicate in and kill tumor cells sparing normal cells are a promising cancer therapy. Traditionally, recombinant HSV vectors have been generated ...