Browsing Harvard Medical School by Keyword "Biotechnology"
Now showing items 1-20 of 132
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3D Multi-isotope Imaging Mass Spectrometry Reveals Penetration of \(^{18}O\)-Trehalose in Mouse Sperm Nucleus
(Public Library of Science, 2012)The prevalence of genetically engineered mice in medical research has led to ever increasing storage costs. Trehalose has a significant beneficial effect in preserving the developmental potential of mouse sperm following ... -
Acellular Bi-Layer Silk Fibroin Scaffolds Support Tissue Regeneration in a Rabbit Model of Onlay Urethroplasty
(Public Library of Science, 2014)Acellular scaffolds derived from Bombyx mori silk fibroin were investigated for their ability to support functional tissue regeneration in a rabbit model of urethra repair. A bi-layer silk fibroin matrix was fabricated by ... -
An Adjustable Gas-Mixing Device to Increase Feasibility of In Vitro Culture of Plasmodium falciparum Parasites in the Field
(Public Library of Science, 2014)A challenge to conducting high-impact and reproducible studies of the mechanisms of P. falciparum drug resistance, invasion, virulence, and immunity is the lack of robust and sustainable in vitro culture in the field. While ... -
Adult Cardiac Progenitor Cell Aggregates Exhibit Survival Benefit Both In Vitro and In Vivo
(Public Library of Science, 2012)Background: A major hurdle in the use of exogenous stems cells for therapeutic regeneration of injured myocardium remains the poor survival of implanted cells. To date, the delivery of stem cells into myocardium has largely ... -
Aminoimidazole Carboxamide Ribonucleotide (AICAR) Inhibits the Growth of Retinoblastoma In Vivo by Decreasing Angiogenesis and Inducing Apoptosis
(Public Library of Science, 2013)5-Aminoimidazole-4-carboxamide-1-β-4-ribofuranoside (AICAR), an analog of AMP is widely used as an activator of AMP-kinase (AMPK), a protein that regulates the responses of the cell to energy change. Recently, we showed ... -
Analysis of Somatic Microsatellite Indels Identifies Driver Events in Human Tumors
(Springer Science and Business Media LLC, 2017-09-11)Microsatellites (MSs) are tracts of variable-length repeats of short DNA motifs that exhibit high rates of mutation in the form of insertions or deletions (indels) of the repeated motif. Despite their prevalence, the ... -
Anion-Sensitive Fluorophore Identifies the Drosophila Swell-Activated Chloride Channel in a Genome-Wide RNA Interference Screen
(Public Library of Science, 2012)When cells swell in hypo-osmotic solutions, chloride-selective ion channels (\(Cl_{swell}\)) activate to reduce intracellular osmolality and prevent catastrophic cell rupture. Despite intensive efforts to assign a molecular ... -
Antigen identification and high-throughput interaction mapping by reprogramming viral entry
(Springer Science and Business Media LLC, 2022-04)Deciphering immune recognition is critical to understanding a broad range of diseases, and to develop effective vaccines and immunotherapies. Efforts to do so are limited by a lack of technologies capable of simultaneously ... -
Automated neuron tracking inside moving and deforming C. elegans using deep learning and targeted augmentation
(Springer Science and Business Media LLC, 2023-12-05)Recent advances allow sustained rapid 3D functional imaging of large numbers of neurons inside behaving animals. To decode neural activity, individual neurons must be segmented and tracked. This can be challenging inside ... -
Binary DNA Nanostructures for Data Encryption
(Public Library of Science, 2012)We present a simple and secure system for encrypting and decrypting information using DNA self-assembly. Binary data is encoded in the geometry of DNA nanostructures with two distinct conformations. Removing or leaving out ... -
Biological Time Series Analysis Using a Context Free Language: Applicability to Pulsatile Hormone Data
(Public Library of Science, 2014)We present a novel approach for analyzing biological time-series data using a context-free language (CFL) representation that allows the extraction and quantification of important features from the time-series. This ... -
Biomarker Profiling by Nuclear Magnetic Resonance Spectroscopy for the Prediction of All-Cause Mortality: An Observational Study of 17,345 Persons
(Public Library of Science, 2014)Background: Early identification of ambulatory persons at high short-term risk of death could benefit targeted prevention. To identify biomarkers for all-cause mortality and enhance risk prediction, we conducted high-throughput ... -
Biophysical Prediction of Protein-Peptide Interactions and Signaling Networks Using Machine Learning
(Springer Science and Business Media LLC, 2020-01-06)In mammalian cells, much of signal transduction is mediated by weak protein-protein interactions between globular peptide-binding domains (PBDs) and unstructured peptidic motifs in partner proteins. The number and diversity ... -
Capturing sequence diversity in metagenomes with comprehensive and scalable probe design
(Springer Science and Business Media LLC, 2019-02)Metagenomic sequencing has the potential to transform microbial detection and characterization, but new tools are needed to improve its sensitivity. Here we present CATCH, a computational method to enhance nucleic-acid ... -
Catecholate Siderophores Protect Bacteria from Pyochelin Toxicity
(Public Library of Science, 2012)Background: Bacteria produce small molecule iron chelators, known as siderophores, to facilitate the acquisition of iron from the environment. The synthesis of more than one siderophore and the production of multiple ... -
Cell-free biosensors for rapid detection of water contaminants
(Springer Science and Business Media LLC, 2020-07-06)Access to safe drinking water is a global worldwide, and methods to reliably and easily detect contaminants could be transformative. We report the development of a cell-free in vitro transcription system that uses RNA ... -
Challenges in Using Cultured Primary Rodent Hepatocytes or Cell Lines to Study Hepatic HDL Receptor SR-BI Regulation by Its Cytoplasmic Adaptor PDZK1
(Public Library of Science, 2013)Background: PDZK1 is a four PDZ-domain containing cytoplasmic protein that binds to a variety of membrane proteins via their C-termini and can influence the abundance, localization and/or function of its target proteins. ... -
Chip-Based Comparison of the Osteogenesis of Human Bone Marrow- and Adipose Tissue-Derived Mesenchymal Stem Cells under Mechanical Stimulation
(Public Library of Science, 2012)Adipose tissue-derived stem cells (ASCs) are considered as an attractive stem cell source for tissue engineering and regenerative medicine. We compared human bone marrow-derived mesenchymal stem cells (hMSCs) and hASCs ... -
Clinicians’ Contributions to the Development of Coronary Artery Stents: A Qualitative Study of Transformative Device Innovation
(Public Library of Science, 2014)Background: Medical device innovation remains poorly understood, and policymakers disagree over how to incentivize early development. We sought to elucidate the components of transformative health care innovation by ... -
Cloud-Enabled Microscopy and Droplet Microfluidic Platform for Specific Detection of Escherichia coli in Water
(Public Library of Science, 2014)We report an all-in-one platform – ScanDrop – for the rapid and specific capture, detection, and identification of bacteria in drinking water. The ScanDrop platform integrates droplet microfluidics, a portable imaging ...