Now showing items 1-8 of 8

    • Characterization of Mammalian Selenoprotein O: A Redox-Active Mitochondrial Protein 

      Han, Seong-Jeong; Lee, Byung Cheon; Yim, Sun Hee; Gladyshev, Vadim N.; Lee, Seung-Rock (Public Library of Science, 2014)
      Selenoproteins exhibit diverse biological functions, most of which are associated with redox control. However, the functions of approximately half of mammalian selenoproteins are not known. One such protein is Selenoprotein ...
    • Differential Disruption of EWS-FLI1 Binding by DNA-Binding Agents 

      Chen, Changmin; Wonsey, Diane R.; Lemieux, Madeleine E.; Kung, Andrew L. (Public Library of Science, 2013)
      Fusion of the EWS gene to FLI1 produces a fusion oncoprotein that drives an aberrant gene expression program responsible for the development of Ewing sarcoma. We used a homogenous proximity assay to screen for compounds ...
    • Ferristatin II Promotes Degradation of Transferrin Receptor-1 In Vitro and In Vivo 

      Byrne, Shaina L.; Buckett, Peter D.; Kim, Jonghan; Luo, Flora; Sanford, Jack; Chen, Juxing; Enns, Caroline; Wessling-Resnick, Marianne (Public Library of Science, 2013)
      Previous studies have shown that the small molecule iron transport inhibitor ferristatin (NSC30611) acts by down-regulating transferrin receptor-1 (TfR1) via receptor degradation. In this investigation, we show that another ...
    • A Homogeneous, High-Throughput Assay for Phosphatidylinositol 5-Phosphate 4-Kinase with a Novel, Rapid Substrate Preparation 

      Davis, Mindy I.; Sasaki, Atsuo T.; Shen, Min; Emerling, Brooke M.; Thorne, Natasha; Michael, Sam; Pragani, Rajan; Boxer, Matthew; Sumita, Kazutaka; Takeuchi, Koh; Auld, Douglas S.; Li, Zhuyin; Cantley, Lewis C.; Simeonov, Anton (Public Library of Science, 2013)
      Phosphoinositide kinases regulate diverse cellular functions and are important targets for therapeutic development for diseases, such as diabetes and cancer. Preparation of the lipid substrate is crucial for the development ...
    • A New Class of Small Molecule Inhibitor of BMP Signaling 

      Sanvitale, Caroline E.; Kerr, Georgina; Chaikuad, Apirat; Ramel, Marie-Christine; Mohedas, Agustin Humberto; Reichert, Sabine; Wang, You; Triffitt, James T.; Cuny, Gregory D.; Yu, Paul B.; Hill, Caroline S.; Bullock, Alex N. (Public Library of Science, 2013)
      Growth factor signaling pathways are tightly regulated by phosphorylation and include many important kinase targets of interest for drug discovery. Small molecule inhibitors of the bone morphogenetic protein (BMP) receptor ...
    • Production of α-Galactosylceramide by a Prominent Member of the Human Gut Microbiota 

      Wieland Brown, Laura C.; Penaranda, Cristina; Kashyap, Purna C.; Williams, Brianna B.; Clardy, Jon; Kronenberg, Mitchell; Sonnenburg, Justin L.; Comstock, Laurie E.; Bluestone, Jeffrey A.; Fischbach, Michael A. (Public Library of Science, 2013)
      While the human gut microbiota are suspected to produce diffusible small molecules that modulate host signaling pathways, few of these molecules have been identified. Species of Bacteroides and their relatives, which often ...
    • Studies of Dynamic Protein-Protein Interactions in Bacteria Using Renilla Luciferase Complementation Are Undermined by Nonspecific Enzyme Inhibition 

      Hatzios, Stavroula-Artemis K.; Ringgaard, Simon; Davis, Brigid; Waldor, Matthew K. (Public Library of Science, 2012)
      The luciferase protein fragment complementation assay is a powerful tool for studying protein-protein interactions. Two inactive fragments of luciferase are genetically fused to interacting proteins, and when these two ...
    • Three-dimensional Structure of Victorivirus HvV190S Suggests Coat Proteins in Most Totiviruses Share a Conserved Core 

      Dunn, Sarah E.; Li, Hua; Cardone, Giovanni; Nibert, Max Lee; Ghabrial, Said A.; Baker, Timothy S. (Public Library of Science, 2013)
      Double-stranded (ds)RNA fungal viruses are currently assigned to six different families. Those from the family Totiviridae are characterized by nonsegmented genomes and single-layer capsids, 300–450 Å in diameter. ...