Browsing Harvard Medical School by FAS Department "Molecular and Cellular Biology"
Now showing items 1-17 of 17
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Attenuation of Notch and Hedgehog Signaling Is Required for Fate Specification in the Spinal Cord
(Public Library of Science, 2012)During the development of the spinal cord, proliferative neural progenitors differentiate into postmitotic neurons with distinct fates. How cells switch from progenitor states to differentiated fates is poorly understood. ... -
A BioBrick compatible strategy for genetic modification of plants
(BioMed Central, 2012)Background: Plant biotechnology can be leveraged to produce food, fuel, medicine, and materials. Standardized methods advocated by the synthetic biology community can accelerate the plant design cycle, ultimately making ... -
Chromatin- and Transcription-Related Factors Repress Transcription from within Coding Regions throughout the Saccharomyces cerevisiae Genome
(Public Library of Science, 2008)Previous studies in Saccharomyces cerevisiae have demonstrated that cryptic promoters within coding regions activate transcription in particular mutants. We have performed a comprehensive analysis of cryptic transcription ... -
Discovery of 3-Formyl-Tyrosine Metabolites from Pseudoalteromonas tunicata through Heterologous Expression
(American Chemical Society, 2009)Genome mining and identification of natural product gene clusters typically relies on the presence of canonical nonribosomal polypeptide synthetase (NRPS) or polyketide synthase (PKS) domains. Recently, other condensation ... -
Distinct Spatiotemporal Activity in Principal Neurons of the Mouse Olfactory Bulb in Anesthetized and Awake States
(Frontiers Media S.A., 2013)The acquisition of olfactory information and its early processing in mammals are modulated by brain states through sniffing behavior and neural feedback. We imaged the spatiotemporal pattern of odor-evoked activity in a ... -
Evolutionary Genomics Reveals Lineage-Specific Gene Loss and Rapid Evolution of a Sperm-Specific Ion Channel Complex: CatSpers and CatSperβ
(Public Library of Science, 2008)The mammalian CatSper ion channel family consists of four sperm-specific voltage-gated Ca2+ channels that are crucial for sperm hyperactivation and male fertility. All four CatSper subunits are believed to assemble into a ... -
Galactose Metabolism Plays a Crucial Role in Biofilm Formation by Bacillus subtilis
(American Society of Microbiology, 2012)Galactose is a common monosaccharide that can be utilized by all living organisms via the activities of three main enzymes that make up the Leloir pathway: GalK, GalT, and GalE. In Bacillus subtilis, the absence of GalE ... -
Genetic Reporter System for Positioning of Proteins at the Bacterial Pole
(American Society of Microbiology, 2012)Spatial organization within bacteria is fundamental to many cellular processes, although the basic mechanisms underlying localization of proteins to specific sites within bacteria are poorly understood. The study of protein ... -
Genetically Encoded Calcium Indicators for Ulti-Color Neural Activity Imaging and Combination With Optogenetics
(Frontiers Media S.A., 2013)Genetically encoded calcium indicators (GECIs) are powerful tools for systems neuroscience. Here we describe red, single-wavelength GECIs, “RCaMPs,” engineered from circular permutation of the thermostable red fluorescent ... -
A Genome-Wide Screen to Identify Transcription Factors Expressed in Pelvic Ganglia of the Lower Urinary Tract
(Frontiers Research Foundation, 2012)Relative positions of neurons within mature murine pelvic ganglia based on expression of neurotransmitters have been described. However the spatial organization of developing innervation in the murine urogenital tract (UGT) ... -
Harnessing RNA interference to prevent or treat HSV-2 and HIV
(BioMed Central, 2006) -
A Keystone for ncRNA
(BioMed Central, 2012)A report on the Keystone symposium 'Non-coding RNAs' held at Snowbird, Utah, USA, 31 March to 5 April 2012. -
The MEK2-binding tumor suppressor hDlg is recruited by E-cadherin to the midbody ring
(BioMed Central, 2011)Background: The human homologue of the Drosophila Discs-large tumor suppressor protein, hDlg, is a multi-domain cytoplasmic protein that localizes to the membrane at intercellular junction sites. At both synaptic junctions ... -
Modeling Infectious Disease in Mice: Co-Adaptation and the Role of Host-Specific IFNγ Responses
(Public Library of Science, 2009) -
A Polycomb complex remains bound through DNA replication in the absence of other eukaryotic proteins
(Nature Publishing Group, 2012)Propagation of chromatin states through DNA replication is central to epigenetic regulation and can involve recruitment of chromatin proteins to replicating chromatin through interactions with replication fork components. ... -
A Polycomb Group Protein Is Retained at Specific Sites on Chromatin in Mitosis
(Public Library of Science, 2012)Epigenetic regulation of gene expression, including by Polycomb Group (PcG) proteins, may depend on heritable chromatin states, but how these states can be propagated through mitosis is unclear. Using immunofluorescence ... -
Structural and Biochemical Consequences of Disease-Causing Mutations in the Ankyrin Repeat Domain of the Human TRPV4 Channel
(American Chemical Society, 2012)The TRPV4 calcium-permeable cation channel plays important physiological roles in osmosensation, mechanosensation, cell barrier formation, and bone homeostasis. Recent studies reported that mutations in TRPV4, including ...