A Chemical-Genetic Strategy Reveals Distinct Temporal Requirements for SAD-1 Kinase in Neuronal Polarization and Synapse Formation

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A Chemical-Genetic Strategy Reveals Distinct Temporal Requirements for SAD-1 Kinase in Neuronal Polarization and Synapse Formation

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Title: A Chemical-Genetic Strategy Reveals Distinct Temporal Requirements for SAD-1 Kinase in Neuronal Polarization and Synapse Formation
Author: Kim, Joanne SM; Zhang, Chao; Shokat, Kevan M; Zhen, Mei; Lilley, Brendan Neil; Sanes, Joshua R.

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Citation: Kim, Joanne SM, Brendan N. Lilley, Chao Zhang, Kevan M. Shokat, Joshua R. Sanes, and Mei Zhen. 2008. A chemical-genetic strategy reveals distinct temporal requirements for SAD-1 kinase in neuronal polarization and synapse formation. Neural Development 3:23.
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Abstract: Background: Neurons assemble into a functional network through a sequence of developmental processes including neuronal polarization and synapse formation. In Caenorhabditis elegans, the serine/threonine SAD-1 kinase is essential for proper neuronal polarity and synaptic organization. To determine if SAD-1 activity regulates the establishment or maintenance of these neuronal structures, we examined its temporal requirements using a chemical-genetic method that allows for selective and reversible inactivation of its kinase activity in vivo. Results: We generated a PP1 analog-sensitive variant of SAD-1. Through temporal inhibition of SAD-1 kinase activity we show that its activity is required for the establishment of both neuronal polarity and synaptic organization. However, while SAD-1 activity is needed strictly when neurons are polarizing, the temporal requirement for SAD-1 is less stringent in synaptic organization, which can also be re-established during maintenance. Conclusion: This study reports the first temporal analysis of a neural kinase activity using the chemical-genetic system. It reveals that neuronal polarity and synaptic organization have distinct temporal requirements for SAD-1.
Published Version: doi:10.1186/1749-8104-3-23
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2564922/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Open Access Policy Articles, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#OAP
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:4455249

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  • FAS Scholarly Articles [7176]
    Peer reviewed scholarly articles from the Faculty of Arts and Sciences of Harvard University
 
 

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