A Chemical-Genetic Strategy Reveals Distinct Temporal Requirements for SAD-1 Kinase in Neuronal Polarization and Synapse Formation
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A Chemical-Genetic Strategy Reveals Distinct Temporal Requirements for SAD-1 Kinase in Neuronal Polarization and Synapse Formation
| Title: | A Chemical-Genetic Strategy Reveals Distinct Temporal Requirements for SAD-1 Kinase in Neuronal Polarization and Synapse Formation |
| Author: |
Kim, Joanne SM; Zhang, Chao; Shokat, Kevan M; Zhen, Mei; Lilley, Brendan Neil; Sanes, Joshua R.
Note: Order does not necessarily reflect citation order of authors. |
| Citation: | Kim, Joanne SM, Brendan N. Lilley, Chao Zhang, Kevan M. Shokat, Joshua R. Sanes, and Mei Zhen. 2008. A chemical-genetic strategy reveals distinct temporal requirements for SAD-1 kinase in neuronal polarization and synapse formation. Neural Development 3:23. |
| Full Text & Related Files: |
2564922.pdf (1.195Mb; PDF) 
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| Abstract: | Background: Neurons assemble into a functional network through a sequence of developmental processes including neuronal polarization and synapse formation. In Caenorhabditis elegans, the serine/threonine SAD-1 kinase is essential for proper neuronal polarity and synaptic organization. To determine if SAD-1 activity regulates the establishment or maintenance of these neuronal structures, we examined its temporal requirements using a chemical-genetic method that allows for selective and reversible inactivation of its kinase activity in vivo. Results: We generated a PP1 analog-sensitive variant of SAD-1. Through temporal inhibition of SAD-1 kinase activity we show that its activity is required for the establishment of both neuronal polarity and synaptic organization. However, while SAD-1 activity is needed strictly when neurons are polarizing, the temporal requirement for SAD-1 is less stringent in synaptic organization, which can also be re-established during maintenance. Conclusion: This study reports the first temporal analysis of a neural kinase activity using the chemical-genetic system. It reveals that neuronal polarity and synaptic organization have distinct temporal requirements for SAD-1. |
| Published Version: | doi:10.1186/1749-8104-3-23 |
| Other Sources: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2564922/pdf/ |
| Terms of Use: | This article is made available under the terms and conditions applicable to Open Access Policy Articles, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#OAP |
| Citable link to this page: | http://nrs.harvard.edu/urn-3:HUL.InstRepos:4455249 |
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