Pairing of Competitive and Topologically Distinct Regulatory Modules Enhances Patterned Gene Expression

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Pairing of Competitive and Topologically Distinct Regulatory Modules Enhances Patterned Gene Expression

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dc.contributor.author Yanai, Itai
dc.contributor.author Baugh, L Ryan
dc.contributor.author Shen-Orr, Shai S
dc.contributor.author Claggett, Julia M
dc.contributor.author Slonim, Donna K
dc.contributor.author Smith, Jessica J.
dc.contributor.author Roehrig, Casey
dc.contributor.author Hill, Andrew A.
dc.contributor.author Hunter, Craig P.
dc.date.accessioned 2010-10-04T17:26:36Z
dc.date.issued 2008
dc.identifier.citation Yanai, Itai, L. Ryan Baugh, Jessica J. Smith, Casey Roehrig, Shai S. Shen-Orr, Julia M. Claggett, Andrew A. Hill, Donna K. Slonim, and Craig P Hunter. 2008. Pairing of competitive and topologically distinct regulatory modules enhances patterned gene expression. Molecular Systems Biology 4:163. en_US
dc.identifier.issn 1744-4292 en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:4455975
dc.description.abstract Biological networks are inherently modular, yet little is known about how modules are assembled to enable coordinated and complex functions. We used RNAi and time series, whole-genome microarray analyses to systematically perturb and characterize components of a Caenorhabditis elegans lineage-specific transcriptional regulatory network. These data are supported by selected reporter gene analyses and comprehensive yeast one-hybrid and promoter sequence analyses. Based on these results, we define and characterize two modules composed of muscle- and epidermal-specifying transcription factors that function together within a single cell lineage to robustly specify multiple cell types. The expression of these two modules, although positively regulated by a common factor, is reliably segregated among daughter cells. Our analyses indicate that these modules repress each other, and we propose that this cross-inhibition coupled with their relative time of induction function to enhance the initial asymmetry in their expression patterns, thus leading to the observed invariant gene expression patterns and cell lineage. The coupling of asynchronous and topologically distinct modules may be a general principle of module assembly that functions to potentiate genetic switches. en_US
dc.description.sponsorship Molecular and Cellular Biology en_US
dc.language.iso en_US en_US
dc.publisher Nature Publishing Group en_US
dc.relation.isversionof doi:10.1038/msb.2008.6 en_US
dc.relation.hasversion http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2267734/pdf/ en_US
dash.license LAA
dc.subject functional genomics en_US
dc.subject development en_US
dc.subject C. elegans en_US
dc.subject gene regulatory network en_US
dc.subject module en_US
dc.subject transcription factor en_US
dc.title Pairing of Competitive and Topologically Distinct Regulatory Modules Enhances Patterned Gene Expression en_US
dc.type Journal Article en_US
dc.description.version Version of Record en_US
dc.relation.journal Molecular Systems Biology en_US
dash.depositing.author Hunter, Craig P.
dc.date.available 2010-10-04T17:26:36Z

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  • FAS Scholarly Articles [7106]
    Peer reviewed scholarly articles from the Faculty of Arts and Sciences of Harvard University

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