Fibroblast-Derived Induced Pluripotent Stem Cells Show No Common Retroviral Vector Insertions

DSpace/Manakin Repository

Fibroblast-Derived Induced Pluripotent Stem Cells Show No Common Retroviral Vector Insertions

Citable link to this page

. . . . . .

Title: Fibroblast-Derived Induced Pluripotent Stem Cells Show No Common Retroviral Vector Insertions
Author: Varas, Florencio; de Andres-Aguayo, Luisa; di Tullio, Alessandro; Pantano, Lorena; Notredame, Cedric; Graf, Thomas; Stadtfeld, Matthias; Maherali, Nimet Alia; Hochedlinger, Konrad

Note: Order does not necessarily reflect citation order of authors.

Citation: Varas, Florencio, Matthias Stadtfeld, Luisa de Andres-Aguayo, Nimet Maherali, Alessandro di Tullio, Lorena Pantano, Cedric Notredame, et al. 2009. Fibroblast-Derived Induced Pluripotent Stem Cells Show No Common Retroviral Vector Insertions. Stem Cells 27(2): 300-306.
Full Text & Related Files:
Abstract: Several laboratories have reported the reprogramming of mouse and human fibroblasts into pluripotent cells, using retroviruses carrying the Oct4, Sox2, Klf4, and c-Myc transcription factor genes. In these experiments the frequency of reprogramming was lower than 0.1% of the infected cells, raising the possibility that additional events are required to induce reprogramming, such as activation of genes triggered by retroviral insertions. We have therefore determined by ligation-mediated polymerase chain reaction (LM-PCR) the retroviral insertion sites in six induced pluripotent stem (iPS) cell clones derived from mouse fibroblasts. Seventy-nine insertion sites were assigned to a single mouse genome location. Thirty-five of these mapped to gene transcription units, whereas 29 insertions landed within 10 kilobases of transcription start sites. No common insertion site was detected among the iPS clones studied. Moreover, bioinformatics analyses revealed no enrichment of a specific gene function, network, or pathway among genes targeted by retroviral insertions. We conclude that Oct4, Sox2, Klf4, and c-Myc are sufficient to promote fibroblast-to-iPS cell reprogramming and propose that the observed low reprogramming frequencies may have alternative explanations.
Published Version: doi:10.1634/stemcells.2008-0696
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2729671/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Open Access Policy Articles, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#OAP
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:4459933

Show full Dublin Core record

This item appears in the following Collection(s)

  • FAS Scholarly Articles [7501]
    Peer reviewed scholarly articles from the Faculty of Arts and Sciences of Harvard University
 
 

Search DASH


Advanced Search
 
 

Submitters