Reactivity-Dependent PCR: Direct, Solution-Phase in Vitro Selection for Bond Formation

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Reactivity-Dependent PCR: Direct, Solution-Phase in Vitro Selection for Bond Formation

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Title: Reactivity-Dependent PCR: Direct, Solution-Phase in Vitro Selection for Bond Formation
Author: Gorin, David Joel; Kamlet, Adam Seth; Liu, David Ruchien

Note: Order does not necessarily reflect citation order of authors.

Citation: Gorin, David J., Adam S. Kamlet, and David R. Liu. 2009. Reactivity-Dependent PCR: Direct, Solution-Phase Selection for Bond Formation. Journal of the American Chemical Society 131: 9189-9191.
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Abstract: In vitro selection is a key component of efforts to discover functional nucleic acids and small molecules from libraries of DNA, RNA, and DNA-encoded small molecules. Such selections have been widely used to evolve RNA and DNA catalysts and, more recently, to discover new reactions from DNA-encoded libraries of potential substrates. While effective, current strategies for selections of bond-forming and bond-cleaving reactivity are generally indirect, require the synthesis of biotin-linked substrates, and involve multiple solution-phase and solid-phase manipulations. In this work we report the successful development and validation of reactivity-dependent PCR (RDPCR), a new method that more directly links bond formation or bond cleavage with the amplification of desired sequences and that obviates the need for solid-phase capture, washing, and elution steps. We show that RDPCR can be used to select for bond formation in the context of reaction discovery and for bond cleavage in the context of protease activity profiling.
Published Version: doi:10.1021/ja903084a
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2710857/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Open Access Policy Articles, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#OAP
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:4459985

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  • FAS Scholarly Articles [7594]
    Peer reviewed scholarly articles from the Faculty of Arts and Sciences of Harvard University
 
 

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