Structure of Clathrin Coat with Bound Hsc70 and Auxilin: Mechanism of Hsc70-Facilitated Disassembly

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Structure of Clathrin Coat with Bound Hsc70 and Auxilin: Mechanism of Hsc70-Facilitated Disassembly

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Title: Structure of Clathrin Coat with Bound Hsc70 and Auxilin: Mechanism of Hsc70-Facilitated Disassembly
Author: Böcking, Till; Grigorieff, Nikolaus; Xing, Yi; Wolf, Matthias; Kirchhausen, Tomas Leopold; Harrison, Stephen C.

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Citation: Xing, Yi, Till Böcking, Matthias Wolf, Nikolaus Grigorieff, Tomas Kirchhausen, and Stephen C. Harrison. 2010. Structure of clathrin coat with bound Hsc70 and auxilin: Mechanism of Hsc70-facilitated disassembly. EMBO Journal 29(3): 655-665.
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Abstract: The chaperone Hsc70 drives the clathrin assembly–disassembly cycle forward by stimulating dissociation of a clathrin lattice. A J-domain containing co-chaperone, auxilin, associates with a freshly budded clathrin-coated vesicle, or with an in vitro assembled clathrin coat, and recruits Hsc70 to its specific heavy-chain-binding site. We have determined by electron cryomicroscopy (cryoEM), at about 11 Å resolution, the structure of a clathrin coat (in the D6-barrel form) with specifically bound Hsc70 and auxilin. The Hsc70 binds a previously analysed site near the C-terminus of the heavy chain, with a stoichiometry of about one per three-fold vertex. Its binding is accompanied by a distortion of the clathrin lattice, detected by a change in the axial ratio of the D6 barrel. We propose that when Hsc70, recruited to a position close to its target by the auxilin J-domain, splits ATP, it clamps firmly onto its heavy-chain site and locks in place a transient fluctuation. Accumulation of the local strain thus imposed at multiple vertices can then lead to disassembly.
Published Version: doi:10.1038/emboj.2009.383
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2830701/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:4460783

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  • FAS Scholarly Articles [6948]
    Peer reviewed scholarly articles from the Faculty of Arts and Sciences of Harvard University
 
 

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