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Title:	Identification of a Distinct Class of Cytoskeleton-Associated mRNAs Using Microarray Technology
Author:	Huang, Sui; Brock, Amy Monique Lepre; Ingber, Donald Elliott Note: Order does not necessarily reflect citation order of authors.
Citation:	Brock, Amy, Sui Huang, and Donald E Ingber. 2003. Identification of a distinct class of cytoskeleton-associated mRNAs using microarray technology. BMC Cell Biology 4:6.
Full Text & Related Files:	167255.pdf (552.2Kb; PDF)
Abstract:	Background: Interactions between mRNA and the cytoskeleton are critical for the localization of a number of transcripts in eukaryotic somatic cells. To characterize additional transcripts that may be subject to this form of regulation, we developed a two-step approach that utilizes biochemical fractionation of cells to isolate transcripts from different subcellular compartments followed by microarray analysis to examine and compare these subpopulations of transcripts in a massively-parallel manner. Results: Using this approach, mRNA was extracted from the cytoskeleton-rich and the cytosolic fractions of the promyelocytic HL-60 cell line. We identify a subset of 22 transcripts that are significantly enriched in the cytoskeleton-associated population. The majority of these encode structural proteins and/or proteins known to interact with elements of the cytoskeleton. Localization required an intact actin cytoskeleton and was largely conserved upon differentiation of precursor HL-60 cells to a macrophage-like phenotype. Conclusions: We conclude that the association of transcripts with the actin cytoskeleton in somatic cells may be a critical post-transcriptional regulatory event that controls a larger class of genes than has previously been recognized.
Published Version:	doi:10.1186/1471-2121-4-6
Other Sources:	http://www.ncbi.nlm.nih.gov/pmc/articles/PMC167255/pdf/
Terms of Use:	This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page:	http://nrs.harvard.edu/urn-3:HUL.InstRepos:4460825

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