Identification of a Distinct Class of Cytoskeleton-Associated mRNAs Using Microarray Technology
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| dc.contributor.author |
Huang, Sui |
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| dc.contributor.author |
Brock, Amy Monique Lepre
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| dc.contributor.author |
Ingber, Donald Elliott
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| dc.date.accessioned |
2010-10-06T20:06:24Z |
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| dc.date.issued |
2003 |
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| dc.identifier.citation |
Brock, Amy, Sui Huang, and Donald E Ingber. 2003. Identification of a distinct class of cytoskeleton-associated mRNAs using microarray technology. BMC Cell Biology 4:6. |
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| dc.identifier.issn |
1471-2121 |
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| dc.identifier.uri |
http://nrs.harvard.edu/urn-3:HUL.InstRepos:4460825 |
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| dc.description.abstract |
Background: Interactions between mRNA and the cytoskeleton are critical for the localization of a number of transcripts in eukaryotic somatic cells. To characterize additional transcripts that may be subject to this form of regulation, we developed a two-step approach that utilizes biochemical fractionation of cells to isolate transcripts from different subcellular compartments followed by microarray analysis to examine and compare these subpopulations of transcripts in a massively-parallel manner. Results: Using this approach, mRNA was extracted from the cytoskeleton-rich and the cytosolic fractions of the promyelocytic HL-60 cell line. We identify a subset of 22 transcripts that are significantly enriched in the cytoskeleton-associated population. The majority of these encode structural proteins and/or proteins known to interact with elements of the cytoskeleton. Localization required an intact actin cytoskeleton and was largely conserved upon differentiation of precursor HL-60 cells to a macrophage-like phenotype. Conclusions: We conclude that the association of transcripts with the actin cytoskeleton in somatic cells may be a critical post-transcriptional regulatory event that controls a larger class of genes than has previously been recognized. |
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| dc.description.sponsorship |
Engineering and Applied Sciences |
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| dc.language.iso |
en_US |
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| dc.publisher |
BioMed Central |
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| dc.relation.isversionof |
doi:10.1186/1471-2121-4-6 |
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| dc.relation.hasversion |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC167255/pdf/ |
en_US |
| dash.license |
LAA |
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| dc.title |
Identification of a Distinct Class of Cytoskeleton-Associated mRNAs Using Microarray Technology |
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| dc.type |
Journal Article |
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| dc.description.version |
Version of Record |
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| dc.relation.journal |
BMC Cell Biology |
en_US |
| dash.depositing.author |
Ingber, Donald Elliott
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| dc.date.available |
2010-10-06T20:06:24Z |
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FAS Scholarly Articles [5171]
Peer reviewed scholarly articles from the Faculty of Arts and Sciences of Harvard University
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