Identification of a Distinct Class of Cytoskeleton-Associated mRNAs Using Microarray Technology

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Identification of a Distinct Class of Cytoskeleton-Associated mRNAs Using Microarray Technology

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dc.contributor.author Huang, Sui
dc.contributor.author Brock, Amy Monique Lepre
dc.contributor.author Ingber, Donald Elliott
dc.date.accessioned 2010-10-06T20:06:24Z
dc.date.issued 2003
dc.identifier.citation Brock, Amy, Sui Huang, and Donald E Ingber. 2003. Identification of a distinct class of cytoskeleton-associated mRNAs using microarray technology. BMC Cell Biology 4:6. en_US
dc.identifier.issn 1471-2121 en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:4460825
dc.description.abstract Background: Interactions between mRNA and the cytoskeleton are critical for the localization of a number of transcripts in eukaryotic somatic cells. To characterize additional transcripts that may be subject to this form of regulation, we developed a two-step approach that utilizes biochemical fractionation of cells to isolate transcripts from different subcellular compartments followed by microarray analysis to examine and compare these subpopulations of transcripts in a massively-parallel manner. Results: Using this approach, mRNA was extracted from the cytoskeleton-rich and the cytosolic fractions of the promyelocytic HL-60 cell line. We identify a subset of 22 transcripts that are significantly enriched in the cytoskeleton-associated population. The majority of these encode structural proteins and/or proteins known to interact with elements of the cytoskeleton. Localization required an intact actin cytoskeleton and was largely conserved upon differentiation of precursor HL-60 cells to a macrophage-like phenotype. Conclusions: We conclude that the association of transcripts with the actin cytoskeleton in somatic cells may be a critical post-transcriptional regulatory event that controls a larger class of genes than has previously been recognized. en_US
dc.description.sponsorship Engineering and Applied Sciences en_US
dc.language.iso en_US en_US
dc.publisher BioMed Central en_US
dc.relation.isversionof doi:10.1186/1471-2121-4-6 en_US
dc.relation.hasversion http://www.ncbi.nlm.nih.gov/pmc/articles/PMC167255/pdf/ en_US
dash.license LAA
dc.title Identification of a Distinct Class of Cytoskeleton-Associated mRNAs Using Microarray Technology en_US
dc.type Journal Article en_US
dc.description.version Version of Record en_US
dc.relation.journal BMC Cell Biology en_US
dash.depositing.author Ingber, Donald Elliott
dc.date.available 2010-10-06T20:06:24Z

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  • FAS Scholarly Articles [6948]
    Peer reviewed scholarly articles from the Faculty of Arts and Sciences of Harvard University

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