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dc.contributor.authorMyatt, Theodore A
dc.contributor.authorJohnston, Sebastian L
dc.contributor.authorRudnick, Stephen N.
dc.contributor.authorMilton, Donald Kirby
dc.date.accessioned2010-11-12T15:54:52Z
dc.date.issued2003
dc.identifier.citationMyatt, Theodore A., Sebastian L. Johnston, Stephen Rudnick, and Donald K. Milton. 2003. Airborne rhinovirus detection and effect of ultraviolet irradiation on detection by a semi-nested RT-PCR assay. BMC Public Health 3:5.en_US
dc.identifier.issn1471-2458en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:4553348
dc.description.abstractBackground: Rhinovirus, the most common cause of upper respiratory tract infections, has been implicated in asthma exacerbations and possibly asthma deaths. Although the method of transmission of rhinoviruses is disputed, several studies have demonstrated that aerosol transmission is a likely method of transmission among adults. As a first step in studies of possible airborne rhinovirus transmission, we developed methods to detect aerosolized rhinovirus by extending existing technology for detecting infectious agents in nasal specimens. Methods: We aerosolized rhinovirus in a small aerosol chamber. Experiments were conducted with decreasing concentrations of rhinovirus. To determine the effect of UV irradiation on detection of rhinoviral aerosols, we also conducted experiments in which we exposed aerosols to a UV dose of 684 mJ/m2. Aerosols were collected on Teflon filters and rhinovirus recovered in Qiagen AVL buffer using the Qiagen QIAamp Viral RNA Kit (Qiagen Corp., Valencia, California) followed by semi-nested RT-PCR and detection by gel electrophoresis. Results: We obtained positive results from filter samples that had collected at least 1.3 TCID50 of aerosolized rhinovirus. Ultraviolet irradiation of airborne virus at doses much greater than those used in upper-room UV germicidal irradiation applications did not inhibit subsequent detection with the RT-PCR assay. Conclusion: The air sampling and extraction methodology developed in this study should be applicable to the detection of rhinovirus and other airborne viruses in the indoor air of offices and schools. This method, however, cannot distinguish UV inactivated virus from infectious viral particles.en_US
dc.language.isoen_USen_US
dc.publisherBioMed Centralen_US
dc.relation.isversionofdoi:10.1186/1471-2458-3-5en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC140314/pdf/en_US
dash.licenseLAA
dc.titleAirborne Rhinovirus Detection and Effect of Ultraviolet Irradiation on Detection by a Semi-Nested RT-PCR Assayen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalBMC Public Healthen_US
dash.depositing.authorRudnick, Stephen N.
dc.date.available2010-11-12T15:54:52Z
dash.affiliation.otherSPH^Exposure Epidemiology and Risk Programen_US
dash.affiliation.otherSPH^Exposure Epidemiology and Risk Programen_US
dc.identifier.doi10.1186/1471-2458-3-5*
dash.contributor.affiliatedRudnick, Stephen
dash.contributor.affiliatedMilton, Donald


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