Generation of Mice with a Conditional Foxp2 Null Allele

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Generation of Mice with a Conditional Foxp2 Null Allele

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dc.contributor.author French, Catherine A
dc.contributor.author Groszer, Matthias
dc.contributor.author Preece, Christopher
dc.contributor.author Coupe, Anne-Marie
dc.contributor.author Fisher, Simon E
dc.contributor.author Rajewsky, Klaus
dc.date.accessioned 2010-12-09T14:57:58Z
dc.date.issued 2007
dc.identifier.citation French, Catherine A., Matthias Groszer, Christopher Preece, Anne-Marie Coupe, Klaus Rajewsky, and Simon E. Fisher. 2007. Generation of mice with a conditional null allele. Genesis 45(7): 440-446. en_US
dc.identifier.issn 1526-954X en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:4621601
dc.description.abstract Disruptions of the human FOXP2 gene cause problems with articulation of complex speech sounds, accompanied by impairment in many aspects of language ability. The FOXP2/Foxp2 transcription factor is highly similar in humans and mice, and shows a complex conserved expression pattern, with high levels in neuronal subpopulations of the cortex, striatum, thalamus, and cerebellum. In the present study we generated mice in which loxP sites flank exons 12–14 of Foxp2; these exons encode the DNA-binding motif, a key functional domain. We demonstrate that early global Cre-mediated recombination yields a null allele, as shown by loss of the loxP-flanked exons at the RNA level and an absence of Foxp2 protein. Homozygous null mice display severe motor impairment, cerebellar abnormalities and early postnatal lethality, consistent with other Foxp2 mutants. When crossed to transgenic lines expressing Cre protein in a spatially and/or temporally controlled manner, these conditional mice will provide new insights into the contributions of Foxp2 to distinct neural circuits, and allow dissection of roles during development and in the mature brain. genesis 45:440–446, 2007. Published 2007 Wiley-Liss, Inc. en_US
dc.language.iso en_US en_US
dc.publisher Wiley-Blackwell en_US
dc.relation.isversionof doi: 10.1002/dvg.20305 en_US
dc.relation.hasversion http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2682329/pdf/ en_US
dash.license META_ONLY
dc.subject Foxp2 en_US
dc.subject conditional null allele en_US
dc.subject Sox2-Cre en_US
dc.subject speech and language en_US
dc.subject brain development en_US
dc.title Generation of Mice with a Conditional Foxp2 Null Allele en_US
dc.type Journal Article en_US
dc.description.version Version of Record en_US
dc.relation.journal Genesis -- New York- Wiley Liss- en_US
dash.depositing.author Rajewsky, Klaus
dash.embargo.until 10000-01-01
dash.affiliation.other HMS^Pathology en_US
dash.affiliation.other HMS^Pediatrics-Children's Hospital en_US

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