A Case Study of the Reproducibility of Transcriptional Reporter Cell-Based RNAi Screens in Drosophila

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A Case Study of the Reproducibility of Transcriptional Reporter Cell-Based RNAi Screens in Drosophila

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Title: A Case Study of the Reproducibility of Transcriptional Reporter Cell-Based RNAi Screens in Drosophila
Author: DasGupta, Ramanuj; Booker, Matthew; Mathey-Prevot, Bernard; Gonsalves, Foster; Changkakoty, Binita; Nybakken, Kent E.; Perrimon, Norbert

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Citation: DasGupta, Ramanuj, Kent Nybakken, Matthew Booker, Bernard Mathey-Prevot, Foster Gonsalves, Binita Changkakoty, and Norbert Perrimon. 2007. A case study of the reproducibility of transcriptional reporter cell-based RNAi screens in Drosophila. Genome Biology 8(9): R203.
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Abstract: Off-target effects have been demonstrated to be a major source of false-positives in RNA interference (RNAi) high-throughput screens. In this study, we re-assess the previously published transcriptional reporter-based whole-genome RNAi screens for the Wingless and Hedgehog signaling pathways using second generation double-stranded RNA libraries. Furthermore, we investigate other factors that may influence the outcome of such screens, including cell-type specificity, robustness of reporters, and assay normalization, which determine the efficacy of RNAi-knockdown of target genes.
Published Version: doi:10.1186/gb-2007-8-9-r203
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2375041/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:4632531

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