dc.contributor.author | Lee, Vincent T. | |
dc.contributor.author | Matewish, Jody M | |
dc.contributor.author | Kessler, Jennifer L | |
dc.contributor.author | Hyodo, Mamoru | |
dc.contributor.author | Hayakawa, Yoshihiro | |
dc.contributor.author | Lory, Stephen | |
dc.date.accessioned | 2010-12-21T19:14:23Z | |
dc.date.issued | 2007 | |
dc.identifier.citation | Lee, Vincent T., Jody M. Matewish, Jennifer L. Kessler, Mamoru Hyodo, Yoshihiro Hayakawa, and Stephen Lory. 2007. A cyclic-di-GMP receptor required for bacterial exopolysaccharide production. Molecular Microbiology 65(6): 1474-1484. | en_US |
dc.identifier.issn | 0950-382X | en_US |
dc.identifier.uri | http://nrs.harvard.edu/urn-3:HUL.InstRepos:4632771 | |
dc.description.abstract | Bis-(3′,5′)-cyclic-dimeric-guanosine monophosphate (c-di-GMP) has been shown to be a global regulatory molecule that modulates the reciprocal responses of bacteria to activate either virulence pathways or biofilm formation. The mechanism of c-di-GMP signal transduction, including recognition of c-di-GMP and subsequent phenotypic regulation, remain largely uncharacterized. The key components of these regulatory pathways are the various adaptor proteins (c-di-GMP receptors). There is compelling evidence suggesting that, in addition to PilZ domains, there are other unidentified c-di-GMP receptors. Here we show that the PelD protein of Pseudomonas aeruginosa is a novel c-di-GMP receptor that mediates c-di-GMP regulation of PEL polysaccharide biosynthesis. Analysis of PelD orthologues identified a number of conserved residues that are required for c-di-GMP binding as well as synthesis of the PEL polysaccharide. Secondary structure similarities of PelD to the inhibitory site of diguanylate cyclase suggest that a common fold can act as a platform to bind c-di-GMP. The combination of a c-di-GMP binding site with a variety of output signalling motifs within one protein domain provides an explanation for the specificity for different cellular responses to this regulatory dinucleotide.d | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Blackwell Publishing Ltd | en_US |
dc.relation.isversionof | doi:10.1111/j.1365-2958.2007.05879.x | en_US |
dc.relation.hasversion | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2170427/pdf/ | en_US |
dash.license | LAA | |
dc.title | A Cyclic-di-GMP Receptor Required for Bacterial Exopolysaccharide Production | en_US |
dc.type | Journal Article | en_US |
dc.description.version | Version of Record | en_US |
dc.relation.journal | Molecular Microbiology | en_US |
dash.depositing.author | Lory, Stephen | |
dc.date.available | 2010-12-21T19:14:23Z | |
dash.affiliation.other | HMS^Microbiology and Molecular Genetics | en_US |
dc.identifier.doi | 10.1111/j.1365-2958.2007.05879.x | * |
dash.contributor.affiliated | Lory, Stephen | |