Unique Sequence Features of the Human Adenovirus 31 Complete Genomic Sequence are Conserved in Clinical Isolates

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Unique Sequence Features of the Human Adenovirus 31 Complete Genomic Sequence are Conserved in Clinical Isolates

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dc.contributor.author Hofmayer, Soeren
dc.contributor.author Darr, Sebastian
dc.contributor.author Rehren, Fabienne
dc.contributor.author Heim, Albert
dc.contributor.author Madisch, Ijad
dc.date.accessioned 2010-12-22T14:46:19Z
dc.date.issued 2009
dc.identifier.citation Hofmayer, Soeren, Ijad Madisch, Sebastian Darr, Fabienne Rehren, and Albert Heim. 2009. Unique sequence features of the Human Adenovirus 31 complete genomic sequence are conserved in clinical isolates. BMC Genomics 10:557. en_US
dc.identifier.issn 1471-2164 en_US
dc.identifier.uri http://nrs.harvard.edu/urn-3:HUL.InstRepos:4633184
dc.description.abstract Background: Human adenoviruses (HAdV) are causing a broad spectrum of diseases. One of the most severe forms of adenovirus infection is a disseminated disease resulting in significant morbidity and mortality. Several reports in recent years have identified HAdV-31 from species A (HAdV-A31) as a cause of disseminated disease in children following haematopoetic stem cell transplantation (hSCT) and liver transplantation. We sequenced and analyzed the complete genome of the HAdV-A31 prototype strain to uncover unique sequence motifs associated with its high virulence. Moreover, we sequenced coding regions known to be essential for tropism and virulence (early transcription units E1A, E3, E4, the fiber knob and the penton base) of HAdV-A31 clinical isolates from patients with disseminated disease. Results: The genome size of HAdV-A31 is 33763 base pairs (bp) in length with a GC content of 46.36%. Nucleotide alignment to the closely related HAdV-A12 revealed an overall homology of 84.2%. The genome organization into early, intermediate and late regions is similar to HAdV-A12. Sequence analysis of the prototype strain showed unique sequence features such as an immunoglobulin-like domain in the species A specific gene product E3 CR1 beta and a potentially integrin binding RGD motif in the C-terminal region of the protein IX. These features were conserved in all analyzed clinical isolates. Overall, amino acid sequences of clinical isolates were highly conserved compared to the prototype (99.2 to 100%), but a synonymous/non synonymous ratio (S/N) of 2.36 in E3 CR1 beta suggested positive selection. Conclusion: Unique sequence features of HAdV-A31 may enhance its ability to escape the host's immune surveillance and may facilitate a promiscuous tropism for various tissues. Moderate evolution of clinical isolates did not indicate the emergence of new HAdV-A31 subtypes in the recent years. en_US
dc.language.iso en_US en_US
dc.publisher BioMed Central en_US
dc.relation.isversionof doi:10.1186/1471-2164-10-557 en_US
dc.relation.hasversion http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2794291/pdf/ en_US
dash.license LAA
dc.title Unique Sequence Features of the Human Adenovirus 31 Complete Genomic Sequence are Conserved in Clinical Isolates en_US
dc.type Journal Article en_US
dc.description.version Version of Record en_US
dc.relation.journal BMC Genomics en_US
dash.depositing.author Madisch, Ijad
dc.date.available 2010-12-22T14:46:19Z
dash.affiliation.other HMS^Radiology-Massachusetts General Hospital en_US

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