Physical Responses of Bacterial Chemoreceptors

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Physical Responses of Bacterial Chemoreceptors

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Title: Physical Responses of Bacterial Chemoreceptors
Author: Berg, Howard; Vaknin, Ady

Note: Order does not necessarily reflect citation order of authors.

Citation: Vaknin, Ady and Howard C. Berg. 2007. Physical responses of bacterial chemoreceptors. Journal of Molecular Biology 366, no. 5: 1416-1423.
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Abstract: Chemoreceptors of the bacterium Escherichia coli are thought to form trimers of homodimers that undergo conformational changes upon ligand binding and thereby signal a cytoplasmic kinase. We monitored the physical responses of trimers in living cells lacking other chemotaxis proteins by fluorescently tagging receptors and measuring changes in fluorescence anisotropy. These changes were traced to changes in energy transfer between fluorophores on different dimers of a trimer: attractants move these fluorophores farther apart, and repellents move them closer together. These measurements allowed us to define the responses of bare receptor oligomers to ligand binding and compare them to the corresponding response in kinase activity. Receptor responses could be fit by a simple "two-state" model in which receptor dimers are in either active or inactive conformations, from which energy bias and dissociation constants could be estimated. Comparison with responses in kinase-activity indicated that higher-order interactions are dominant in receptor clusters.
Published Version: http://dx.doi.org/10.1016/j.jmb.2006.12.024
Other Sources: http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=1849981&blobtype=pdf
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:4696185

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  • FAS Scholarly Articles [6463]
    Peer reviewed scholarly articles from the Faculty of Arts and Sciences of Harvard University
 
 

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